| The retinoblastoma protein, RB, is required for gastrointestinal endocrine cells to exit the cell cycle, but not for hormone expression. | |
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MedLine Citation:
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PMID: 17936268 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Important functions of the RB family proteins include inhibition of cell cycle progression and regulation of terminal differentiation. We have examined the role of RB and the related protein, p107, in regulating cell cycle activity and differentiation of gastrointestinal endocrine cells, a relatively quiescent cell population, by conditionally disrupting the RB gene in neurogenin3 (Ngn3)-expressing cells in both p107(+/+) and p107(-/-) mice. Endocrine cells in the small intestine, colon, pancreas, and stomach were present in normal numbers in RB and RB-p107 mutants except for an increase in serotonin cells and decrease in ghrelin cells in the antral stomach. Deletion of RB resulted in a dramatic increase in proliferating serotonin cells in the antral stomach and intestine, whereas other enteroendocrine cell types exhibited much lower cell cycle activity or remained quiescent. The related p107 protein appears dispensable for enteroendocrine differentiation and does not functionally compensate for the loss of RB. Our results suggest that RB is required for enteroendocrine cells, particularly serotonin cells, to undergo cell cycle arrest as they terminally differentiate. RB has relatively subtle effects on enteroendocrine cell differentiation and is not required for the expression of the normal repertoire of hormones in the gastrointestinal tract. |
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Authors:
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Yang Wang; Subir K Ray; Philip W Hinds; Andrew B Leiter |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2007-09-07 |
Journal Detail:
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Title: Developmental biology Volume: 311 ISSN: 1095-564X ISO Abbreviation: Dev. Biol. Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-11-12 Completed Date: 2008-03-24 Revised Date: 2010-12-03 |
Medline Journal Info:
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Nlm Unique ID: 0372762 Medline TA: Dev Biol Country: United States |
Other Details:
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Languages: eng Pagination: 478-86 Citation Subset: IM |
Affiliation:
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Division of Gastroenterology, GRASP Digestive Disease Center, Tufts-New England Medical Center, Boston, MA 02111, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Basic Helix-Loop-Helix Transcription Factors / genetics, metabolism Cell Cycle / physiology* Cell Differentiation / physiology* Cells, Cultured Enteroendocrine Cells / cytology, metabolism* Gastrointestinal Hormones / metabolism* Gastrointestinal Tract / cytology* Ghrelin / metabolism Humans Mice Mice, Knockout Nerve Tissue Proteins / genetics, metabolism Retinoblastoma Protein / genetics, metabolism* Retinoblastoma-Like Protein p107 / genetics, metabolism Serotonin / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AG20208/AG/NIA NIH HHS; DK43673/DK/NIDDK NIH HHS; DK52870/DK/NIDDK NIH HHS; DK67166/DK/NIDDK NIH HHS; P30 DK034928-229002/DK/NIDDK NIH HHS; P30DK34928/DK/NIDDK NIH HHS; R01 AG020208-06/AG/NIA NIH HHS; R01 DK043673-14/DK/NIDDK NIH HHS; R01 DK043673-15/DK/NIDDK NIH HHS; R01 DK067166-03/DK/NIDDK NIH HHS; R01 DK067166-04/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Basic Helix-Loop-Helix Transcription Factors; 0/Gastrointestinal Hormones; 0/Ghrelin; 0/Nerve Tissue Proteins; 0/Neurog3 protein, mouse; 0/Retinoblastoma Protein; 0/Retinoblastoma-Like Protein p107; 50-67-9/Serotonin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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