Document Detail

The retinal anatomy and function of the myelin mutant taiep rat.
MedLine Citation:
PMID:  12573523     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To study the histology and the physiological function of the retina in the neurological myelin mutant, taiep rats during the postnatal developmental period (P20-P360). METHODS: Electroretinography (ERG) was applied to evaluate intensity dependence and spectral sensitivity of the responses to light. Retinal histology, morphometry, and immunocytochemistry were used to characterize the structure of the retina, with particular emphasis on the Müller (glial) cells. RESULTS: In the taiep rats of all ages studied, the scotopic ERG showed normal a- and b-wave amplitudes and latencies; likewise, the scotopic spectral sensitivity function was the same for control and taiep animals, with a maximal sensitivity (lambda(max)) at 500 nm. However, in adult taiep rats (P90 to P360) a secondary cornea-positive wave ('b(2)') was observed in response to high stimulus intensities, which never occurred in controls. This correlated with the observation that in the photopic ERG responses of the taiep rats, the b-wave was reduced in amplitude, and was followed by a rapid cornea-negative after-potential. After 1 year of life, in taiep rats the outer plexiform layer (OPL) became slightly thinner and the inner plexiform/ganglion cell layers (IPL/GCL) appeared to be swollen, and increased in thickness; in addition, the number of retinal neurons (particularly, of photoreceptor cells) slightly decreased. Increased GFAP immunoreactivity revealed a hypertrophy and reactivity of the Müller cells in 1-year-old taiep rats. CONCLUSIONS: The present results suggest the occurrence of a relatively mild and slowly progressing neural retinal alteration in taiep rats, which becomes histologically and functionally evident at the end of the first year of life, and mainly affects the circuit(s) of the photopic ON-response. It is speculated that this alteration is due to missing/altered signals from demyelinated optic nerve.
Andrés E Chávez; Manuel Roncagliolo; Heidrun Kuhrt; Andreas Reichenbach; Adrián G Palacios
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research     Volume:  964     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-02-07     Completed Date:  2003-04-18     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  144-52     Citation Subset:  IM    
Molecular Cellular Center for Neuroscience of Valparaiso, Faculty of Science, University of Valparaíso, P.O. Box 5030, Valparaíso, Chile.
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MeSH Terms
Adaptation, Ocular / physiology
Age Factors
Glial Fibrillary Acidic Protein
Membrane Potentials / physiology
Myelin Sheath / genetics,  metabolism*,  pathology
Nerve Degeneration / genetics,  metabolism*,  pathology
Neuroglia / metabolism*,  pathology
Optic Nerve / growth & development*,  metabolism,  pathology
Photic Stimulation
Photoreceptor Cells / growth & development,  pathology,  physiopathology
Predictive Value of Tests
Rats, Mutant Strains
Retina / growth & development*,  metabolism,  pathology
Retinal Diseases / genetics,  metabolism*,  pathology
Retinal Ganglion Cells / metabolism,  pathology
Reg. No./Substance:
0/Glial Fibrillary Acidic Protein

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