Document Detail


p21(WAF1/Cip1) retards the growth of human squamous cell carcinomas in vivo.
MedLine Citation:
PMID:  9692056     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The excessive proliferation exhibited by cancer cells is frequently a result of their failure to adequately regulate cell cycle progression. In the present study, we developed a xenograft model of oral cancer in athymic mice, using squamous carcinoma cell lines and examined the ability of the cyclin-dependent kinase inhibitor p21 (WAF1/Cip1) to retard tumour growth in vivo, using a retroviral delivery system. Human p21 cDNA was cloned by polymerase chain reaction, expressed, and the encoded protein shown to have biological activity in in vitro kinase assays. Amphotropic retrovirus cultures which expressed recombinant p21 were generated and used to treat established squamous cell carcinoma xenografts. Two weeks following onset of treatment tumours injected with p21 virus producer cells showed a reduction in size between 3- and 10-fold compared with tumours which received control cells which produced control virus alone. The data indicate that recombinant p21 may be of future use for therapeutic intervention in oral cancer.
Authors:
M Cardinali; J Jakus; S Shah; J F Ensley; K C Robbins; W A Yeudall
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Oral oncology     Volume:  34     ISSN:  1368-8375     ISO Abbreviation:  Oral Oncol.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-08-13     Completed Date:  1998-08-13     Revised Date:  2006-04-21    
Medline Journal Info:
Nlm Unique ID:  9709118     Medline TA:  Oral Oncol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  211-8     Citation Subset:  IM    
Affiliation:
Oral and Pharyngeal Cancer Branch, National Institute of Dental Research, Bethesda, MD 20892-4330, USA. cardinali@a1.cber.fda.gov
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MeSH Terms
Descriptor/Qualifier:
Animals
Carcinoma, Squamous Cell / metabolism,  pathology,  therapy*
Cyclin-Dependent Kinase Inhibitor p21
Cyclins / physiology*
Gene Therapy / methods*
Genetic Vectors
Humans
Mice
Mice, Nude
Mouth Neoplasms / metabolism,  pathology,  therapy*
Neoplasm Transplantation
Retroviridae / genetics
Transplantation, Heterologous
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/CDKN1A protein, human; 0/Cdkn1a protein, mouse; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins

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