| The resistance-nodulation-division efflux pump EmhABC influences the production of 2,4-diacetylphloroglucinol in Pseudomonas fluorescens 2P24. | |
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MedLine Citation:
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PMID: 19833777 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The polyketide metabolite 2,4-diacetylphloroglucinol (2,4-DAPG) plays a major role in the biological control of soil-borne plant diseases by Pseudomonas fluorescens 2P24. Two mutants (PM810 and PM820) with increased extracellular accumulation of 2,4-DAPG were isolated using transposon mutagenesis. The disrupted genes in these two mutants shared >80 % identity with the genes of the EmhR-EmhABC resistance-nodulation-division (RND) efflux system of P. fluorescens cLP6a. The deletion of emhA (PM802), emhB (PM803) or emhC (PM804) genes in strain 2P24 increased the extracellular accumulation of 2,4-DAPG, whereas the deletion of the emhR (PM801) gene decreased the biosynthesis of 2,4-DAPG. The promoter assay confirmed the elevated transcription of emhABC in the EmhR disrupted strain (PM801) and an indirect negative regulation of 2,4-DAPG biosynthetic locus transcription by the EmhABC efflux pump. Induction by exogenous 2,4-DAPG led to remarkable differences in transcription of chromosome-borne phlA : : lacZ fusion in PM901 and PM811 (emhA(-)) strains. Additionally, the EmhABC system in strain 2P24 was involved in the resistance to a group of toxic compounds, including ampicillin, chloramphenicol, tetracycline, ethidium bromide and crystal violet. In conclusion, our results suggest that the EmhABC system is an important element that influences the production of antibiotic 2,4-DAPG and enhances resistance to toxic compounds in P. fluorescens 2P24. |
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Authors:
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Tao Tian; Xiao-Gang Wu; Hui-Mei Duan; Li-Qun Zhang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-15 |
Journal Detail:
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Title: Microbiology (Reading, England) Volume: 156 ISSN: 1465-2080 ISO Abbreviation: Microbiology (Reading, Engl.) Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2009-12-23 Completed Date: 2010-02-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9430468 Medline TA: Microbiology Country: England |
Other Details:
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Languages: eng Pagination: 39-48 Citation Subset: IM |
Affiliation:
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Department of Plant Pathology, China Agricultural University, Beijing, 100193, PR China. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/FJ807391 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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genetics,
metabolism* Cloning, Molecular Drug Resistance, Bacterial Gene Deletion Gene Expression Regulation, Bacterial Genetic Complementation Test Membrane Transport Proteins / genetics, metabolism* Microbial Sensitivity Tests Molecular Sequence Data Mutagenesis Mutation Phloroglucinol / analogs & derivatives, metabolism Pseudomonas fluorescens / drug effects, genetics*, metabolism Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Membrane Transport Proteins; 108-73-6/Phloroglucinol; 2161-86-6/2,4-diacetylphloroglucinol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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