Document Detail


The rescuing effect of nerve growth factor is the result of up-regulation of bcl-2 in hyperoxia-induced apoptosis of a subclone of pheochromocytoma cells, PC12h.
MedLine Citation:
PMID:  9302089     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The rat pheochromocytoma cell line PC12 is useful for studying neuronal cell differentiation since this cell line differentiates into neuron-like cells in response to nerve growth factor (NGF). We demonstrated that PC12h cells, a subclone of PC12 cells, died under hyperoxia (50% O2). This cell death did not occur in the presence of antioxidant reagents. In the dead cells, DNA fragmentation and chromatin condensation were observed, suggesting that hyperoxia-induced apoptosis via reactive oxygen species (ROS). NGF effectively suppressed this hyperoxia-induced apoptosis. Accordingly, the amounts of bcl-2, a proto-oncogene product, increased in the cells rescued from apoptosis by NGF. Furthermore, bcl-2 antisense oligonucleotide canceled this rescuing effect of NGF. The present findings indicate that NGF rescues PC12h cells from hyperoxia-induced apoptosis via up-regulation of bcl-2.
Authors:
S Katoh; Y Mitsui; K Kitani; T Suzuki
Related Documents :
7535839 - Lectin binding distinguishes between neuroendocrine and neuronal derivatives of the sym...
20654569 - An in vitro model for the assessment of manganese neurotoxicity.
21984579 - Beta-cell uncoupling protein 2 regulates reactive oxygen species production, which infl...
17287109 - Shp2 in pc12 cells: ngf versus egf signalling.
20939009 - Effects of vibration on differentiation of cultured pc12 cells.
14737019 - Possible role of interleukin-6 in pc12 cell death induced by mpp+ and tetrahydroisoquin...
19264889 - Effects of pirfenidone on proliferation, migration, and collagen contraction of human t...
24874089 - Large scale rnai reveals the requirement of nuclear envelope breakdown for nuclear impo...
18770749 - Reporters of gene expression: autofluorescent proteins.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience letters     Volume:  232     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-11-13     Completed Date:  1997-11-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  71-4     Citation Subset:  IM; S    
Affiliation:
Radiation Safety Office, University of Tokyo Hospital, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*
Cell Survival / drug effects
DNA Fragmentation
Hyperoxia / metabolism*
Nerve Growth Factors / pharmacology*
Oligonucleotides, Antisense / pharmacology
PC12 Cells
Proto-Oncogene Proteins c-bcl-2 / metabolism*
Rats
Up-Regulation / drug effects*
Chemical
Reg. No./Substance:
0/Nerve Growth Factors; 0/Oligonucleotides, Antisense; 0/Proto-Oncogene Proteins c-bcl-2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  [D-Pen2,5]enkephalin and glutamate regulate the expression of delta-opioid receptors in rat cortical...
Next Document:  Alpha2-adrenoreceptor agonist, dexmedetomidine, alters acute gene expression after global ischemia i...