Document Detail

The rescuing effect of nerve growth factor is the result of up-regulation of bcl-2 in hyperoxia-induced apoptosis of a subclone of pheochromocytoma cells, PC12h.
MedLine Citation:
PMID:  9302089     Owner:  NLM     Status:  MEDLINE    
The rat pheochromocytoma cell line PC12 is useful for studying neuronal cell differentiation since this cell line differentiates into neuron-like cells in response to nerve growth factor (NGF). We demonstrated that PC12h cells, a subclone of PC12 cells, died under hyperoxia (50% O2). This cell death did not occur in the presence of antioxidant reagents. In the dead cells, DNA fragmentation and chromatin condensation were observed, suggesting that hyperoxia-induced apoptosis via reactive oxygen species (ROS). NGF effectively suppressed this hyperoxia-induced apoptosis. Accordingly, the amounts of bcl-2, a proto-oncogene product, increased in the cells rescued from apoptosis by NGF. Furthermore, bcl-2 antisense oligonucleotide canceled this rescuing effect of NGF. The present findings indicate that NGF rescues PC12h cells from hyperoxia-induced apoptosis via up-regulation of bcl-2.
S Katoh; Y Mitsui; K Kitani; T Suzuki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience letters     Volume:  232     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-11-13     Completed Date:  1997-11-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  71-4     Citation Subset:  IM; S    
Radiation Safety Office, University of Tokyo Hospital, Japan.
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MeSH Terms
Apoptosis / drug effects*
Cell Survival / drug effects
DNA Fragmentation
Hyperoxia / metabolism*
Nerve Growth Factors / pharmacology*
Oligonucleotides, Antisense / pharmacology
PC12 Cells
Proto-Oncogene Proteins c-bcl-2 / metabolism*
Up-Regulation / drug effects*
Reg. No./Substance:
0/Nerve Growth Factors; 0/Oligonucleotides, Antisense; 0/Proto-Oncogene Proteins c-bcl-2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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