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The reproduction of centrosomes: nuclear versus cytoplasmic controls.
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MedLine Citation:
PMID:  3782286     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The tight coordination normally found between nuclear events and the doubling of centrosomes at each cell cycle suggests that nuclear activities may be part of the mechanism that controls the reproduction of centrosomes. To determine if this is the case, we used a micropipette to completely remove the nucleus from eggs of the sea urchin Lytechinus variegatus at prophase of the first mitosis, leaving only one centrosome in the cell. The subsequent behavior of this centrosome was then followed in vivo with the polarization microscope. In all cases the centrosome reproduced in a precise 1:2:4:8 fashion with a periodicity that was slightly slower than the centrosome cycle of control eggs. The cell cycle-related changes in centrosome morphology were identical to those of control eggs in that: (a) the astral birefringence varied cyclically to a normal extent, (b) the astral focus enlarged and then flattened during the telophase equivalent, (c) cleavage furrows were initiated as the astral birefringence faded, and (d) daughter centrosomes separated before the increase in astral birefringence at the onset of each mitosis. To determine if centrioles also reproduced normally, enucleate eggs were followed in vivo until they contained eight centrosomes. They were then individually removed from the preparations, fixed, and embedded. Each egg was serially 0.25-micron sectioned for observation with the high voltage electron microscope. We completely reconstructed 23 centrosomes in four eggs; all centrosomes contained two centrioles apiece. These results demonstrate that the subunits for complete centrosome assembly can be stockpiled ahead of time and that the properly controlled use of these subunits for centrosome reproduction does not require nuclear transcription or nuclear DNA synthesis at each cell cycle.
Authors:
G Sluder; F J Miller; C L Rieder
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  103     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1986 Nov 
Date Detail:
Created Date:  1987-01-21     Completed Date:  1987-01-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1873-81     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle*
Cell Nucleus / physiology*
Centrioles / physiology*,  ultrastructure
Cytoplasm / physiology*
Mitosis*
Mitotic Spindle Apparatus / ultrastructure
Sea Urchins / embryology*
Grant Support
ID/Acronym/Agency:
GM-30758/GM/NIGMS NIH HHS; P0-30-12708//PHS HHS; RR-02157/RR/NCRR NIH HHS
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 11 Year: 1986
Volume: 103 Issue: 5
First Page: 1873 Last Page: 1881
ID: 2114374
Publisher Id: 87057641
PubMed Id: 3782286

The reproduction of centrosomes: nuclear versus cytoplasmic controls


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