Document Detail

A reproducible laser-wounded skin equivalent model to study the effects of aging in vitro.
MedLine Citation:
PMID:  15312297     Owner:  NLM     Status:  MEDLINE    
Skin aging involves both chronological and photoaging processes. The effects of these processes are often overlapping and include changes in both the stratified epithelium and the fibroblast-rich dermis. Wound healing is frequently delayed with aging and can result in scarring. A skin equivalent model can be used to study the role of cells and the extracellular matrix in the process of wound healing. Current studies using this model employ a full-thickness wound placed atop a nonwounded dermis to mimic a partial-thickness wound. However, a true reproducible partial-thickness wound model has yet to be described. In this study, we investigated whether a laser-wounded skin equivalent would be a useful partial-thickness wound healing model. Three lasers were compared for the ability to generate a reproducible wound: an erbium-YAG, a high-powered excimer, and a low-powered excimer laser. Reepithelialization ability was tested using newborn and adult skin keratinocytes, adult esophageal keratinocytes, and cdk4-overexpressing newborn keratinocytes. Keratinocyte compartmentalization and basement membrane formation were assessed by immunofluorescence. The erbium-YAG and high-powered excimer laser cut reproducible wounds but left the remaining surface either discolored due to thermal damage and/or ragged; keratinocytes were unable to migrate into the wound area. The low-powered excimer laser cut reproducible wounds, leaving the cut surface intact and visibly unaltered; keratinocytes reepithelialized the wound in a collagenase-dependent manner within 3 days; and return of compartmentalization and basement membrane occurred within 14 days. The laser-wounded skin equivalent is an adjustable, reproducible partial-thickness wound model where keratinocyte biology akin to in vivo can be studied, and will be useful to study the effects of aging on wound healing.
Melville B Vaughan; Ruben D Ramirez; Spencer A Brown; Jimmy C Yang; Woodring E Wright; Jerry W Shay
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Rejuvenation research     Volume:  7     ISSN:  1549-1684     ISO Abbreviation:  Rejuvenation Res     Publication Date:  2004  
Date Detail:
Created Date:  2004-08-17     Completed Date:  2004-09-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  101213381     Medline TA:  Rejuvenation Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  99-110     Citation Subset:  IM    
Department of Cell Biology, UT Southwestern Medical Center, Dallas, Texas, USA.
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MeSH Terms
Basement Membrane / metabolism,  radiation effects
Cell Compartmentation / physiology,  radiation effects
Cell Movement / drug effects,  physiology,  radiation effects
Cells, Cultured
Collagen / metabolism
Collagenases / antagonists & inhibitors
Enzyme Inhibitors / pharmacology
Fibroblasts / cytology
Infant, Newborn
Keratinocytes / cytology,  metabolism,  radiation effects*
Protein Precursors / metabolism
Reproducibility of Results
Skin / cytology,  injuries*
Skin Aging / physiology*
Grant Support
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Protein Precursors; 60108-77-2/involucrin; 9007-34-5/Collagen; EC 3.4.24.-/Collagenases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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