Document Detail

A reproducible experimental model of focal cerebral ischemia in the cat.
MedLine Citation:
PMID:  6498494     Owner:  NLM     Status:  MEDLINE    
In the past experimental methods used for producing focal cerebral ischemia have had considerable difficulty with regard to reproducibility of the size of the infarcted region. In this study we have developed an experimental model which enables us to consistently produce focal regions of cerebral ischemia (resulting in infarction) which vary little in size in a number of animals. Thirty-seven cats (3-4 kg b. wt.) anesthetized with chloralose and urethane were used. Physiologic monitoring and adjustments maintained arterial blood values as follows: pCO2 27-35 Torr, pO2 100-150 Torr, pH +/- 7.4, glucose 200 mg%, hematocrit greater than 25. The left middle cerebral artery was exposed via a transorbital approach and occluded for 1-2 h with and without left and/or both carotid artery occlusion. Sixteen hours following the ischemic episode, the animals were sacrificed and sections of fresh brain tissue were processed for vital staining using 1% tetrazolium solution. With this method normal brain areas appear dark red, ischemic regions (without infarction) appear gray and irreversibly infarcted areas appear pinkish-white. The volumetric dimensions of the lesioned area were measured using a planimeter. The same tissue was also evaluated histologically by means of standard histopathologic techniques on paraffin-embedded material. Infarcted areas as delineated macroscopically by the tetrazolium correlated well with the light microscopic findings. Ten animals subjected to a 2-h occlusion of the left middle cerebral artery (LMCA) and both carotid arteries resulted in a reproducible infarct which was 3.2 +/- 0.7 ml in volume. This represents 13.3 +/- 2.9% of the total volume of both cerebral hemispheres (above the level of the inferior colliculus.(ABSTRACT TRUNCATED AT 250 WORDS)
B Bose; J L Osterholm; R Berry
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Brain research     Volume:  311     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  1984 Oct 
Date Detail:
Created Date:  1984-12-28     Completed Date:  1984-12-28     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  385-91     Citation Subset:  IM    
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MeSH Terms
Brain / pathology
Carotid Arteries / physiology
Disease Models, Animal
Ischemic Attack, Transient / physiopathology*
Motor Activity

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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