Document Detail


A reporter gene assay for the detection of phytoestrogens in traditional Chinese medicine.
MedLine Citation:
PMID:  11536376     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bupleurum & Peony Formula (Jia Wei Xiao Yao San) is a herbal formula which possesses a clinical history for the treatment of menopausal syndrome and menstrual irregularity. The present investigation reports the ability to monitor the formula's phytoestrogen content that will allow for the implementation of a standardization protocol that is based on a quantifiable biological response. Utilizing an oestrogen-sensitive chimeric receptor/reporter gene element which has been stably transfected into HeLa cells, the botanical formula was shown to induce the expression of the reporter gene, luciferase, in a dose dependent manner. Pretreatment of the HeLa cells with the botanical formula produced a 5-fold increase in bioluminescence compared with the control. Additionally, our studies showed that the response of the cells, when challenged by the botanical formula, was oestrogen specific. Pretreatment of the cells with tamoxifen effectively blocked the activation of the chimeric oestrogen receptor by the botanical formula. The cell line provides a sensitive assay that can easily detect the presence of phytoestrogens in complex botanical formulas.
Authors:
D M Miller-Martini; R Y Chan; N Y Ip; S J Sheu; Y H Wong
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Phytotherapy research : PTR     Volume:  15     ISSN:  0951-418X     ISO Abbreviation:  Phytother Res     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-09-05     Completed Date:  2002-01-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8904486     Medline TA:  Phytother Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  487-92     Citation Subset:  IM    
Copyright Information:
Copyright 2001 John Wiley & Sons, Ltd.
Affiliation:
The Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
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MeSH Terms
Descriptor/Qualifier:
Adjuvants, Immunologic
Bupleurum / chemistry*
Drugs, Chinese Herbal / chemistry*,  pharmacology
Estradiol / pharmacology
Estrogens, Non-Steroidal / isolation & purification*
Genes, Reporter
Hela Cells
Humans
Isoflavones*
Luciferases / genetics,  metabolism
Luminescent Measurements
Medicine, Chinese Traditional
Menopause / drug effects*
Phytoestrogens
Phytotherapy
Plant Extracts / chemistry
Plant Preparations
Receptors, Estrogen / genetics*,  metabolism
Sensitivity and Specificity
Tamoxifen / pharmacology
Transfection
Chemical
Reg. No./Substance:
0/Adjuvants, Immunologic; 0/Drugs, Chinese Herbal; 0/Estrogens, Non-Steroidal; 0/Isoflavones; 0/Phytoestrogens; 0/Plant Extracts; 0/Plant Preparations; 0/Receptors, Estrogen; 10540-29-1/Tamoxifen; 50-28-2/Estradiol; EC 1.13.12.-/Luciferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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