Document Detail


A report of the 1997, 1998 and 1999 Paternity Testing Workshops of the English Speaking Working Group of the International Society for Forensic Genetics.
MedLine Citation:
PMID:  11118749     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We present the results of the 1997, 1998 and 1999 Paternity Testing Workshops of the English Speaking Working Group of the International Society for Forensic Genetics. The numbers of participating laboratories were 24 (1997), 31 (1998) and 32 (1999). In 1997, all laboratories drew the correct conclusion that the alleged father was the biological father of the child. In 1998, the alleged father was the biological brother of the child and all laboratories excluded him. The scenario in 1999 was a deficiency case consisting of mother, child and the parents of the alleged father and all but one laboratory drew the correct conclusion.The percentage of laboratories routinely performing variable number of tandem repeat (VNTR) investigations using single locus probes (SLPs) and restriction fragment length polymorphism (RFLP) decreased from 83% in 1997 to 66% in 1999. In the three workshops, more than 90% of the laboratories used polymerase chain reaction (PCR) based systems. In 1999, 80% of the laboratories performing PCR, used commercially available short tandem repeat (STR) kits. Other commonly used systems were HLA and PolyMarker investigated with PCR. Conventional systems and RFLP investigations of VNTRs with multi loci probes (MLPs) were used routinely by approximately 20% of the participating laboratories. All laboratories submitting results in the three workshops used RFLP-based VNTRs or/and PCR based VNTRs/STRs. Inter-laboratory comparisons of the results showed a very high concordance. The overall coefficients of variation between the laboratories of the results of RFLP typing of the commonly used VNTRs D2S44, D7S21, D7S22 and D12S11 were 1.2-1.3%. Consistent results were obtained in the great majority of the systems investigated by PCR and typing errors counted for less than 0.3% of the PCR based results.
Authors:
C Hallenberg; N Morling
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Forensic science international     Volume:  116     ISSN:  0379-0738     ISO Abbreviation:  Forensic Sci. Int.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-01-26     Completed Date:  2001-02-15     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  7902034     Medline TA:  Forensic Sci Int     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  23-33     Citation Subset:  IM    
Affiliation:
Department of Forensic Genetics, Institute of Forensic Medicine, University of Copenhagen, Copenhagen DK-2100, Denmark. charlotte.hallenberg@forensic.ku.dk
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MeSH Terms
Descriptor/Qualifier:
Accreditation
DNA Fingerprinting / methods*,  utilization*
DNA Probes / isolation & purification
Databases as Topic
Emigration and Immigration / legislation & jurisprudence
Genetic Markers
Humans
International Agencies*
Male
Minisatellite Repeats
Paternity*
Pedigree
Polymerase Chain Reaction / utilization
Polymorphism, Restriction Fragment Length
Questionnaires
Reproducibility of Results
Chemical
Reg. No./Substance:
0/DNA Probes; 0/Genetic Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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