| A replication stress-induced synchronization method for Arabidopsis thaliana root meristems. | |
| | |
MedLine Citation:
|
PMID: 21070422 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Synchronized cell cultures are an indispensable tool for the identification and understanding of key regulators of the cell cycle. Nevertheless, the use of cell cultures has its disadvantages, because it represents an artificial system that does not completely mimic the endogenous conditions that occur in organized meristems. Here, we present a new and easy method for Arabidopsis thaliana root tip synchronization by hydroxyurea treatment. A major advantage of the method is the possibility of investigating available Arabidopsis cell-cycle mutants without the need to generate cell cultures. As a proof of concept, the effects of over-expression of a dominant negative allele of the B-type cyclin-dependent kinase CDKB1;1 gene on cell-cycle progression were tested. The previously observed prolonged G₂ phase was confirmed, but was found to be compensated for by a reduced G₁ phase. Furthermore, altered S-phase kinetics indicated a functional role for CDKB1;1 during the replication process. |
| | |
Authors:
|
Toon Cools; Anelia Iantcheva; Sara Maes; Hilde Van den Daele; Lieven De Veylder |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-26 |
Journal Detail:
|
Title: The Plant journal : for cell and molecular biology Volume: 64 ISSN: 1365-313X ISO Abbreviation: Plant J. Publication Date: 2010 Nov |
Date Detail:
|
Created Date: 2010-11-12 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9207397 Medline TA: Plant J Country: England |
Other Details:
|
Languages: eng Pagination: 705-14 Citation Subset: IM |
Copyright Information:
|
© 2010 The Authors. The Plant Journal © 2010 Blackwell Publishing Ltd. |
Affiliation:
|
Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Transcriptional silencing induced by Arabidopsis T-DNA mutants is associated with 35S promoter siRNA...
Next Document: Influence of cryptic population structure on observed mating patterns in the wild progenitor of maiz...