Document Detail


The repeatability of superovulatory response and embryo recovery in sheep.
MedLine Citation:
PMID:  11467510     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Over an 8-year period, a total of 328 Scottish Blackface donor ewes were involved in a MOET program. They were synchronized with fluorogestone acetate sponges and superovulated with ovine FSH. After the onset of estrus, ewes were hand-mated and laparoscopic artificial insemination was performed with fresh semen 44-46 h after sponge removal. Embryos were recovered semi-laparoscopically on either Day 5 or Day 6 after insemination. Of the 328 donor ewes used, 222 ewes were supervoulated only once, while the remaining ewes were superovulated either twice (73 ewes), 3 times (26 ewes) or 4 times (7 ewes) at yearly intervals to generate a maximum of 474 records for subsequent analysis. There was no significant change in either mean ovulation rate or the mean number of embryos recovered per donor ewe at successive treatments. However, significant (P < 0.05 at least) effects of both year and donor ewe age existed for superovulatory response and number of embryos recovered, though only the effect of year was significant (P < 0.001) for percentage embryo recovery. Repeatability was significant (P < 0.05 at least) for both superovulatory response (r = 0.55, s.e. 0.055) and number of embryos recovered (r = 0.38, SE 0.074), but not for percentage embryo recovery (r = 0.04, SE 0.102).
Authors:
F Bari; M Khalid; B Wolf; W Haresign; A Murray; B Merrel
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Theriogenology     Volume:  56     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-07-24     Completed Date:  2001-09-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  147-55     Citation Subset:  IM    
Affiliation:
Institute of Rural Studies, University of Wales Aberystwyth, Ceredigion, UK.
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Embryo Transfer / veterinary*
Estrus Synchronization
Female
Flurogestone Acetate / administration & dosage
Follicle Stimulating Hormone / administration & dosage
Insemination, Artificial / veterinary
Laparoscopy / veterinary
Male
Pregnancy
Retrospective Studies
Sheep / embryology,  physiology*
Superovulation / physiology*
Chemical
Reg. No./Substance:
2529-45-5/Flurogestone Acetate; 9002-68-0/Follicle Stimulating Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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