Document Detail


A reliable preparation of mon-dispersed chromosome suspensions for flow cytometry.
MedLine Citation:
PMID:  7085351     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A reproducible, simple and rapid protocol was developed to prepare mono-dispersed chromosome suspensions for flow cytometric analysis. The procedure basically employes: 1. twice rinsing of monolayer cultures to eliminate dead cells and cellular debris, 2. mild fixation of mitotic cells with 1% acetic acid, and 3. ultra sonic treatment to release single metaphase chromosomes. The procedure takes less than 30 min. Isolated chromosomes are storable over months at 4 degrees C. Despite mild fixation many biochemical studies and experiments applied on such fixed chromosomes purified and enriched using electronic sorting are feasible: 1. gene and restriction mapping, 2. cloning of specific gene sequences, and 3. gene frequency analysis.
Authors:
M Stoehr; K J Hutter; M Frank; K Goerttler
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Histochemistry     Volume:  74     ISSN:  0301-5564     ISO Abbreviation:  Histochemistry     Publication Date:  1982  
Date Detail:
Created Date:  1982-08-07     Completed Date:  1982-08-07     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0411300     Medline TA:  Histochemistry     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  57-61     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Fractionation / methods*
Cells, Cultured
Chromosomes* / analysis*
Cricetinae
DNA / analysis
Flow Cytometry*
Mesocricetus
Mitosis
Ultrasonics
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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