Document Detail


A reliable isotope dilution method for simultaneous determination of fumonisins B1, B2 and B3 in traditional Chinese medicines by ultra-high-performance liquid chromatography-tandem mass spectrometry.
MedLine Citation:
PMID:  20730841     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A reliable isotope dilution method for simultaneous determination of fumonisin B1, fumonisin B2 and fumonisin B3 in traditional Chinese medicines by ultra-high-performance LC-MS/MS was developed, and a special focus was placed on the optimization of extraction, cleanup, ultra-high-performance LC separation and MS/MS conditions. Homogenized samples were extracted by 50% acetonitrile aqueous solution and purified with MultiSep 211 Fum columns. A linear gradient mobile phase, consisting of water containing 0.2% formic acid and acetonitrile/methanol (50:50 v/v) and an Acquity UPLC HSS T3 column (100 mm×2.1 mm, 1.8 μm) were employed to obtain the best resolution of the analytes for the positive ESI(+) analysis. The established method was validated by determining the linearity (R(2)≥0.9991), sensitivity (LOQ, 0.08-0.16 ng/mL), recovery (88.2-113.3%) and precision (RSD≤12.3%). Finally, the validated method was successfully applied to the determination of fumonisins in different traditional Chinese medicines. Of 35 samples, 18 were contaminated with fumonisins. The mean levels (incidence) of fumonisin B1, fumonisin B1 and fumonisin B1 in positive samples were 8.55 (94.4%), 4.88 (77.8%) and 0.52 μg/kg (33.3%), respectively. Based on the contaminant situations, a possible association between the contamination levels and the selected herbs was clarified in this study.
Authors:
Zheng Han; Yiping Ren; Xuesong Liu; Lianjun Luan; Yongjiang Wu
Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Journal of separation science     Volume:  33     ISSN:  1615-9314     ISO Abbreviation:  J Sep Sci     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-16     Completed Date:  2011-01-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088554     Medline TA:  J Sep Sci     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2723-33     Citation Subset:  IM    
Affiliation:
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P. R. China.
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MeSH Terms
Descriptor/Qualifier:
Carcinogens, Environmental / analysis
Chromatography, High Pressure Liquid / instrumentation,  methods*
Fumonisins / analysis*
Humans
Medicine, Chinese Traditional*
Molecular Structure
Radioisotope Dilution Technique*
Tandem Mass Spectrometry / instrumentation,  methods*
Chemical
Reg. No./Substance:
0/Carcinogens, Environmental; 0/Fumonisins; 116355-83-0/fumonisin B1; 116355-84-1/fumonisin B2; 136379-59-4/fumonisin B3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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