Document Detail


A reliable and cost-effective in vitro assay of skin viability for skin banks and burn centers.
MedLine Citation:
PMID:  8951547     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The tetrazolium salt, MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide), was used to evaluate the viability of fresh native human skin and cryopreserved human skin under a wide variety of conditions. Viability of fresh and cryopreserved skin was determined with our modification of the MTT assay, and compared with the well-described tetrazolium reductase assay. The MTT assay provides a precise and reproducible index of viability for both fresh and cryopreserved skin. In comparison, the tetrazolium reductase assay (1) is subject to higher levels of variability and, (2) underestimates the viability of fresh native skin. The precision, simplicity, and economy of the MTT assay support its utility in the routine assessment of skin viability in skin banks, burn centers, and skin biology research units.
Authors:
M B Klein; D Shaw; S Barese; G A Chapo; C B Cuono
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of burn care & rehabilitation     Volume:  17     ISSN:  0273-8481     ISO Abbreviation:  J Burn Care Rehabil     Publication Date:    1996 Nov-Dec
Date Detail:
Created Date:  1997-03-13     Completed Date:  1997-03-13     Revised Date:  2006-08-15    
Medline Journal Info:
Nlm Unique ID:  8110188     Medline TA:  J Burn Care Rehabil     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  565-70     Citation Subset:  IM    
Affiliation:
Yale Burn Center & Skin Bank, Yale University School of Medicine, New Haven, CT 06520-8041, USA.
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MeSH Terms
Descriptor/Qualifier:
Burn Units
Cadaver
Cost-Benefit Analysis
Cryopreservation
Culture Techniques
Humans
Sensitivity and Specificity
Skin*
Skin Transplantation
Tetrazolium Salts / diagnostic use*,  pharmacology
Tissue Banks*
Tissue Preservation* / methods
Chemical
Reg. No./Substance:
0/Tetrazolium Salts

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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