| The reliability of urease tests, histology and culture in the diagnosis of Campylobacter pylori infection. | |
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MedLine Citation:
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PMID: 2479086 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The accuracy of various staining techniques for immersion microscopy, of five media for culture and five urease test modalities for the detection of Campylobacter pylori infection is reported. It was found that 2% urea unbuffered gel preparation is the most accurate urease test (sensitivity: 89%, specificity: 98%) but a minimal amount of 10,000 CFU/ml is necessary to observe positivity and the sensitivity of urease tests drops to 52% in patients under antimicrobial treatment. For histological diagnosis, modified Giemsa staining was shown to be slightly superior to H&E. The most valuable diagnosis technique is culture when the biopsy specimen is transported and processed under appropriate conditions. A 94% sensitivity rate was observed with 'BCC agar', a new medium containing brain heart infusion, activated charcoal and horse serum. |
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Authors:
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M Deltenre; Y Glupczynski; C De Prez; J F Nyst; A Burette; M Labbé; C Jonas; E DeKoster |
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Publication Detail:
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Type: Comparative Study; Journal Article; Review |
Journal Detail:
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Title: Scandinavian journal of gastroenterology. Supplement Volume: 160 ISSN: 0085-5928 ISO Abbreviation: Scand. J. Gastroenterol. Suppl. Publication Date: 1989 |
Date Detail:
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Created Date: 1989-12-07 Completed Date: 1989-12-07 Revised Date: 2008-02-13 |
Medline Journal Info:
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Nlm Unique ID: 0437034 Medline TA: Scand J Gastroenterol Suppl Country: NORWAY |
Other Details:
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Languages: eng Pagination: 19-24 Citation Subset: IM |
Affiliation:
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Department of Gastroenterology, Microbiology and Pathology, University Hospital Brugmann ULB-VUB, Brussels, Belgium. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteriological Techniques Campylobacter / enzymology Campylobacter Infections / diagnosis* Clinical Enzyme Tests Histological Techniques Humans Sensitivity and Specificity Staining and Labeling Urease / metabolism |
| Chemical | |
Reg. No./Substance:
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EC 3.5.1.5/Urease |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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