Document Detail


The relevance of ice crystal formation for the cryopreservation of tissues and organs.
MedLine Citation:
PMID:  20159009     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper discusses the role of ice crystal formation in causing or contributing to the difficulties that have been encountered in attempts to develop effective methods for the cryopreservation of some tissues and all organs. It is shown that extracellular ice can be severely damaging but also that cells in situ in tissues can behave quite differently from similar cells in a suspension with respect to intracellular freezing. It is concluded that techniques that avoid the formation of ice altogether are most likely to yield effective methods for the cryopreservation of recalcitrant tissues and vascularised organs.
Authors:
David E Pegg
Related Documents :
3788959 - Kinetic analysis in single, intact cells by microspectrophotometry: evidence for two po...
19766619 - Characterization of cryobiological responses in tf-1 cells using interrupted freezing p...
24105709 - Effects of combined radiofrequency radiation exposure on levels of reactive oxygen spec...
728369 - Energy reserve and cation composition of irreversibly sickled cells in vivo.
18158089 - Principles and practical issues for cryopreservation of nerve cells.
20471379 - Investigating cryoinjury using simulations and experiments. 1: tf-1 cells during two-st...
23175429 - Mir-16-1 plays a role in reducing migration and invasion of glioma cells.
24780819 - Cellular contractility changes are sufficient to drive epithelial scattering.
11161769 - Inhibition of cortisol secretion in dispersed head kidney cells of rainbow trout (oncor...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2010-02-14
Journal Detail:
Title:  Cryobiology     Volume:  60     ISSN:  1090-2392     ISO Abbreviation:  Cryobiology     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-09-06     Completed Date:  2011-01-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  S36-44     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Affiliation:
Department of Biology, University of York, YO10 5YW, UK. biol29@york.ac.uk
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Physiological Phenomena
Cell Survival
Cold Temperature
Cryopreservation / methods*
Cryoprotective Agents
Crystallization*
Freezing
Glycerol
Humans
Ice*
Kidney
Organ Preservation*
Organ Preservation Solutions*
Temperature
Tissue Preservation*
Grant Support
ID/Acronym/Agency:
//Biotechnology and Biological Sciences Research Council; //Medical Research Council
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 0/Ice; 0/Organ Preservation Solutions; 56-81-5/Glycerol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Recombinant expression, purification, and characterization of ThmD, the oxidoreductase component of ...
Next Document:  Inflexin attenuates proinflammatory responses and nuclear factor-kappaB activation in LPS-treated mi...