Document Detail

The relevance of ice crystal formation for the cryopreservation of tissues and organs.
MedLine Citation:
PMID:  20159009     Owner:  NLM     Status:  MEDLINE    
This paper discusses the role of ice crystal formation in causing or contributing to the difficulties that have been encountered in attempts to develop effective methods for the cryopreservation of some tissues and all organs. It is shown that extracellular ice can be severely damaging but also that cells in situ in tissues can behave quite differently from similar cells in a suspension with respect to intracellular freezing. It is concluded that techniques that avoid the formation of ice altogether are most likely to yield effective methods for the cryopreservation of recalcitrant tissues and vascularised organs.
David E Pegg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2010-02-14
Journal Detail:
Title:  Cryobiology     Volume:  60     ISSN:  1090-2392     ISO Abbreviation:  Cryobiology     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-09-06     Completed Date:  2011-01-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  S36-44     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Department of Biology, University of York, YO10 5YW, UK.
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MeSH Terms
Cell Physiological Phenomena
Cell Survival
Cold Temperature
Cryopreservation / methods*
Cryoprotective Agents
Organ Preservation*
Organ Preservation Solutions*
Tissue Preservation*
Grant Support
//Biotechnology and Biological Sciences Research Council; //Medical Research Council
Reg. No./Substance:
0/Cryoprotective Agents; 0/Ice; 0/Organ Preservation Solutions; 56-81-5/Glycerol

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