Document Detail


The release of microparticles by apoptotic cells and their effects on macrophages.
MedLine Citation:
PMID:  16133865     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microparticles are small membrane vesicles released from the cell membrane by exogenous budding. To elucidate the interactions of microparticles with macrophages, the effect of microparticles released from Jurkat T cells on RAW 264.7 cells was determined. Microparticles were isolated by differential centrifugation, using FACS analysis with annexin V and cell surface markers for identification. Various inducers of apoptosis increased the release of microparticles from Jurkat cells up to 5-fold. The released microparticles were then cultured with RAW 264.7 cells. As shown by confocal microscopy and FACS analysis, RAW 264.7 macrophages cleared microparticles by phagocytosis. In addition, microparticles induced apoptosis in RAW 264.7 cells in a dose-dependent manner with up to a 5-fold increase of annexin V positive cells and 9-fold increase in caspase 3 activity. Cell proliferation as determined by the MTT test was also reduced. Furthermore, microparticles stimulated the release of microparticles from macrophages. These effects were specific for macrophages, since no apoptosis was observed in NIH 3T3 and L929 cells. These findings indicate that microparticles can induce macrophages to undergo apoptosis, in turn resulting in a further increase of microparticles. The release of microparticles from apoptotic cells may therefore represent a novel amplification loop of cell death.
Authors:
J H W Distler; L C Huber; A J Hueber; C F Reich; S Gay; O Distler; D S Pisetsky
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Apoptosis : an international journal on programmed cell death     Volume:  10     ISSN:  1360-8185     ISO Abbreviation:  Apoptosis     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-08-31     Completed Date:  2007-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9712129     Medline TA:  Apoptosis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  731-41     Citation Subset:  IM    
Affiliation:
Center of Experimental Rheumatology, Department of Rheumatology, University Hospital Zurich, Zurich, CH-8091, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis*
Caspase 3 / genetics,  metabolism
Coculture Techniques
Formazans / metabolism
Humans
Jurkat Cells
Macrophages / cytology,  enzymology,  metabolism*
Mice
NIH 3T3 Cells
Phagocytosis
Secretory Vesicles / secretion*
Tetrazolium Salts / metabolism
Up-Regulation
Chemical
Reg. No./Substance:
0/Formazans; 0/Tetrazolium Salts; 23305-68-2/MTT formazan; EC 3.4.22.-/Caspase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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