| The release of cytokines by macrophages is not affected by myelin ingestion. | |
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MedLine Citation:
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PMID: 20830806 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Macrophages play an important role in demyelination in multiple sclerosis (MS). Activated macrophages ingest myelin particles, thereby acquiring a foamy appearance. Foamy macrophages in MS lesions were described as being anti-inflammatory. Therefore, these cells might play a role in modulating the inflammatory state of an active lesion. Here, we investigated the mechanism by which myelin uptake leads to skewing of macrophages toward an anti-inflammatory phenotype. Macrophages were incubated with myelin, leading to the development of foamy macrophages. Afterwards, the cells were stimulated with the TLR-4 ligand lipopolysaccharide (LPS), and cytokine production was determined. Interestingly, foamy macrophages appeared to have a reduced cytokine secretion and were LPS insensitive only when generated with one of the myelin preparations. The factor responsible for the different outcomes between different myelin batches turned out to be LPS. We demonstrated that LPS contamination induced insensitivity to LPS in foamy macrophages. On the contrary, foamy macrophages generated in the presence of LPS-free myelin were able to secrete cytokines upon activation. To conclude, myelin-laden macrophages were not LPS insensitive, indicating that they had not acquired an anti-inflammatory phenotype. |
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Authors:
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J E Glim; E J F Vereyken; D A M Heijnen; J J García Vallejo; C D Dijkstra |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Glia Volume: 58 ISSN: 1098-1136 ISO Abbreviation: Glia Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-10-20 Completed Date: 2011-01-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8806785 Medline TA: Glia Country: United States |
Other Details:
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Languages: eng Pagination: 1928-36 Citation Subset: IM |
Affiliation:
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Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Cultured Foam Cells* / immunology, metabolism, pathology Humans Immunophenotyping Interleukin-10 / metabolism*, secretion Lipopolysaccharides / pharmacology Multiple Sclerosis* / immunology, metabolism, pathology Myelin Sheath / immunology, metabolism* Phagocytosis / immunology* Toll-Like Receptor 4 / metabolism Tumor Necrosis Factor-alpha / metabolism*, secretion |
| Chemical | |
Reg. No./Substance:
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0/IL10 protein, human; 0/Lipopolysaccharides; 0/TLR4 protein, human; 0/Toll-Like Receptor 4; 0/Tumor Necrosis Factor-alpha; 130068-27-8/Interleukin-10 |
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