Document Detail


The relative merits of direct morphometry of reconstructions of whole cells, and statistical morphometry by stereology of random sections of cells.
MedLine Citation:
PMID:  1714349     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Stereology, or the derivation of quantitative, three-dimensional (3-D) data about cells by statistical analysis of the structures of random sections, is widely used in cytology and pathology. However, there are situations where this approach is inadequate, and only an analysis of a homogeneous population of whole cells will give the required results. This involved 3-D reconstruction from physical or optical sections, or tomography or photogrammetry of whole-cell mounts. Use of stereo views of individual sections or projections adds considerably to the information available for both contouring and reconstruction. Recent image-processing advances in clinical radiography have shown, for the first time, that rapid, high-resolution digitization and contrast enhancement enable nearly all structural details to be routinely extracted from the micrographs and adequately portrayed. Three-D whole-cell reconstructions provide the digital data for many kinds of morphometric measurements on both whole cells and their individual organelles and membranes. Rapid fixation or freezing allows improved quantitative structure/function correlations of organelles with disturbances in cell metabolism or gene expression.
Authors:
D F Parsons; M Marko; A Leith
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  Cell biophysics     Volume:  17     ISSN:  0163-4992     ISO Abbreviation:  Cell Biophys.     Publication Date:  1990 Dec 
Date Detail:
Created Date:  1991-09-16     Completed Date:  1991-09-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8002185     Medline TA:  Cell Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  227-42     Citation Subset:  IM    
Affiliation:
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
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MeSH Terms
Descriptor/Qualifier:
Animals
Breast Neoplasms / pathology
Cell Division
Cells / cytology*
Computer Graphics
Cytological Techniques*
Cytoskeleton / ultrastructure
Humans
Image Processing, Computer-Assisted
Microscopy, Electron, Scanning
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
RR01219/RR/NCRR NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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