Document Detail

The relationship between urea and pyrimidine de novo synthesis in ruminant liver.
MedLine Citation:
PMID:  3347689     Owner:  NLM     Status:  MEDLINE    
The influence of ammonia and inhibition of the urea cycle by norvaline on orotic acid synthesis in five Polish Merino ewes was investigated. The sheep were exposed to 60 min of successive infusions of 0.9% NaCl, NH4Cl (20 mumol kg-1 min-1), and NH4Cl with DL-norvaline (10 mumol kg-1 min-1) solutions administered to the mesenteric vein. The average rate of orotic acid output, urea output and ammonia uptake in the liver in control conditions (infusion of 0.9% NaCl) was: 1.09 nmol g-1 min-1, 0.97 mumol g-1 min-1 and 0.94 mumol g-1 min-1, respectively. Ammonia significantly stimulated the orotic acid output, urea output and ammonia uptake. At the same time liver uptake of glutamic acid, alanine and glycine increased. Norvaline as a competitive inhibitor of ornithine transcarbamylase depressed citrulline release and urea output in the liver, and elevated, although not significantly, the orotic acid output. It was concluded that ammonia stimulates the de novo synthesis of pyrimidine in the sheep liver but the participation in this effect of carbamyl phosphate generated in the urea cycle is limited.
T Motyl; A Orzechowski; S Pierzynowski
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Quarterly journal of experimental physiology (Cambridge, England)     Volume:  73     ISSN:  0144-8757     ISO Abbreviation:  Q J Exp Physiol     Publication Date:  1988 Jan 
Date Detail:
Created Date:  1988-04-18     Completed Date:  1988-04-18     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8206873     Medline TA:  Q J Exp Physiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1-6     Citation Subset:  IM    
Department of Animal Physiology, Warsaw Agricultural University, Poland.
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MeSH Terms
Amino Acids / metabolism
Ammonia / metabolism
Biological Transport
Liver / metabolism*
Orotic Acid / metabolism
Pyrimidines / biosynthesis*
Sheep / metabolism*
Urea / metabolism*
Reg. No./Substance:
0/Amino Acids; 0/Pyrimidines; 57-13-6/Urea; 65-86-1/Orotic Acid; 7664-41-7/Ammonia

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