Document Detail


The relationship between stem cell seeding efficiency and position in cell cycle.
MedLine Citation:
PMID:  427876     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The seeding efficiency of colony-forming cells from normal, regenerating and velocity-sedimented cycling and non-cycling narrow preparations was compared. Colony-forming cells in cycle were found to exhibit a 50% reduction in splenic seeding when compared to normal marrow or sedimented non-cycling cells. The results of this study indicate that the spleen colony assay underestimates the total number of colony-forming cells by a fraction which is directly related to the number of cells in cycle.
Authors:
F C Monette; J B DeMello
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell and tissue kinetics     Volume:  12     ISSN:  0008-8730     ISO Abbreviation:  Cell Tissue Kinet     Publication Date:  1979 Mar 
Date Detail:
Created Date:  1979-06-11     Completed Date:  1979-06-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0174107     Medline TA:  Cell Tissue Kinet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  161-75     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Marrow / physiology
Cell Cycle*
Colony-Forming Units Assay*
Female
Hematopoietic Stem Cells / cytology*
Mice
Regeneration
Spleen / cytology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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