Document Detail


The relationship between the number of nuclei of an osteoclast and its resorptive capability in vitro.
MedLine Citation:
PMID:  1416078     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study examined the relationship between the number of nuclei in an osteoclast and its resorptive efficiency, as demonstrated by the size of the pit it can make in a mineralized tissue in 24 h in vitro. Osteoclasts released mechanically from prehatch chick long bones were cultured on dentine slices or on plastic dishes for periods of 6 or 24 h. The frequency distribution of the multinucleate tartrate-resistant acid phosphatase (TRAP)-positive cells with different numbers of nuclei was determined: the mean number of nuclei per cell was 6.92, with a mode of 4.47% had 5 or fewer nuclei and only 11% more than 10 nuclei. The pits associated with 292 osteoclasts with known numbers of nuclei were measured using a confocal laser light microscope (Lasertec) and dedicated image analysis system, and depths, plan areas and volumes determined. There was a positive correlation between the number of nuclei per osteoclast and the volume of the pit made, but a trend for the volume resorbed per nucleus to decrease with increase in the number of nuclei per osteoclast.
Authors:
K Piper; A Boyde; S J Jones
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anatomy and embryology     Volume:  186     ISSN:  0340-2061     ISO Abbreviation:  Anat. Embryol.     Publication Date:  1992 Sep 
Date Detail:
Created Date:  1992-11-17     Completed Date:  1992-11-17     Revised Date:  2010-08-25    
Medline Journal Info:
Nlm Unique ID:  7505194     Medline TA:  Anat Embryol (Berl)     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  291-9     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Developmental Biology, University College London, U.K.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Resorption*
Cell Count
Cell Nucleus / ultrastructure*
Chick Embryo
Osteoclasts / physiology,  ultrastructure*
Grant Support
ID/Acronym/Agency:
//Wellcome Trust

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