| The relationship between the number of nuclei of an osteoclast and its resorptive capability in vitro. | |
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MedLine Citation:
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PMID: 1416078 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study examined the relationship between the number of nuclei in an osteoclast and its resorptive efficiency, as demonstrated by the size of the pit it can make in a mineralized tissue in 24 h in vitro. Osteoclasts released mechanically from prehatch chick long bones were cultured on dentine slices or on plastic dishes for periods of 6 or 24 h. The frequency distribution of the multinucleate tartrate-resistant acid phosphatase (TRAP)-positive cells with different numbers of nuclei was determined: the mean number of nuclei per cell was 6.92, with a mode of 4.47% had 5 or fewer nuclei and only 11% more than 10 nuclei. The pits associated with 292 osteoclasts with known numbers of nuclei were measured using a confocal laser light microscope (Lasertec) and dedicated image analysis system, and depths, plan areas and volumes determined. There was a positive correlation between the number of nuclei per osteoclast and the volume of the pit made, but a trend for the volume resorbed per nucleus to decrease with increase in the number of nuclei per osteoclast. |
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Authors:
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K Piper; A Boyde; S J Jones |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Anatomy and embryology Volume: 186 ISSN: 0340-2061 ISO Abbreviation: Anat. Embryol. Publication Date: 1992 Sep |
Date Detail:
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Created Date: 1992-11-17 Completed Date: 1992-11-17 Revised Date: 2010-08-25 |
Medline Journal Info:
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Nlm Unique ID: 7505194 Medline TA: Anat Embryol (Berl) Country: GERMANY |
Other Details:
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Languages: eng Pagination: 291-9 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Developmental Biology, University College London, U.K. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone Resorption* Cell Count Cell Nucleus / ultrastructure* Chick Embryo Osteoclasts / physiology, ultrastructure* |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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