Document Detail


The relationship between motility and the FITC-BSA binding properties of mouse epididymal spermatozoa.
MedLine Citation:
PMID:  6619773     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mouse epididymal spermatozoa exposed to fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) immediately following dilution or after a 2-hour incubation period under fertilization conditions, were assessed by fluorescence microscopy for albumin adsorption. Motile spermatozoa exhibited light fluorescence in the midpiece and tail but not in the head. In contrast the majority of nonmotile spermatozoa displayed a strong and characteristic fluorescence in the post acrosomal region of the sperm head as well as the midpiece. Spermatozoa immobilised by short-term heat stress exhibited fluorescence in the post acrosomal region and midpiece as before but also in the acrosomal cap. The equatorial region failed to fluoresce. The significance of these observations on the involvement of albumin in capacitation is discussed.
Authors:
J D Stanger; P Quinn
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of experimental zoology     Volume:  227     ISSN:  0022-104X     ISO Abbreviation:  J. Exp. Zool.     Publication Date:  1983 Aug 
Date Detail:
Created Date:  1983-11-23     Completed Date:  1983-11-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0375365     Medline TA:  J Exp Zool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  323-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acrosome / metabolism
Animals
Epididymis / cytology
Fluorescein-5-isothiocyanate* / analogs & derivatives*
Fluoresceins / metabolism*
Hot Temperature
Male
Mice
Mice, Inbred Strains
Microscopy, Fluorescence
Serum Albumin / metabolism
Serum Albumin, Bovine / metabolism*
Sperm Capacitation
Sperm Motility*
Spermatozoa / metabolism*
Chemical
Reg. No./Substance:
0/Fluoresceins; 0/Serum Albumin; 0/Serum Albumin, Bovine; 0/fluorescein isothiocyanate bovine serum albumin; 3326-32-7/Fluorescein-5-isothiocyanate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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