Document Detail

The relationship between the glucose uptake system and growth cessation in Candida albicans.
MedLine Citation:
PMID:  7738728     Owner:  NLM     Status:  MEDLINE    
It is thought that dimorphic Candida albicans undergoes changes in its intracellular metabolic state prior to yeast-mycelial transformation. Cells grown in budding form to mid-exponential phase could not be induced to form germ tubes when grown in glucose medium. However, cells in which growth was initially inhibited by either starvation or inhibitors (0.1% hydroxyurea, 4% sodium malonate or 4% 2-deoxy-D-glucose) could be induced to form germ tubes in the same medium. The effects of these initial treatments on the intracellular state in mid-exponential phase cells were analysed by measuring the kinetics of D-glucose uptake. D-glucose uptake in mid-exponential phase and stationary phase cells was measured. The untreated mid-exponential phase cells exhibited only a high Km (6.9 mM). However, mid-exponential phase cells, in which growth was initially inhibited, exhibited both a high Km (3.2-6.2 mM) and a low Km (0.40-0.78 mM) simultaneously. In addition, the stationary phase cells exhibited both a high Km (5.6 mM) and a low Km (0.56 mM). These results suggest that there are two kinetically distinct systems of glucose transport in C. albicans and that changes in the glucose uptake system in C. albicans may be related to intracellular changes prior to transition from the budding to the mycelial form.
T Cho; Y Hagihara; H Kaminishi; K Watanabe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology     Volume:  32     ISSN:  0268-1218     ISO Abbreviation:  J. Med. Vet. Mycol.     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1995-06-08     Completed Date:  1995-06-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8605493     Medline TA:  J Med Vet Mycol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  461-6     Citation Subset:  IM    
Department of Oral Microbiology, Fukuoka Dental College, Japan.
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MeSH Terms
Candida albicans / cytology,  growth & development,  metabolism*
Cell Division / physiology
Deoxyglucose / metabolism
Glucose / metabolism*
Growth Inhibitors / metabolism
Hydroxyurea / metabolism
Malonates / metabolism
Reg. No./Substance:
0/Growth Inhibitors; 0/Malonates; 127-07-1/Hydroxyurea; 154-17-6/Deoxyglucose; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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