| The relationship between collagen scaffold cross-linking agents and neutrophils in the foreign body reaction. | |
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MedLine Citation:
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PMID: 20828809 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order to get more insight into the role of neutrophils on the micro-environment and consequently on macrophages in the foreign body reaction in mice, we investigated the fate of the two differently cross-linked dermal sheep collagen disks (glutaraldehyde = GDSC, hexamethylenediisocyanate = HDSC) in mice implanted in one anatomical location, namely subcutaneously. In GDSC massive infiltration of neutrophils is seen at day 2 and day 21, whereas in HDSC only minor infiltration is seen at day 2. The presence of neutrophils coincided with high levels of IFN-γ, a cytokine that activates macrophages. Major differences were seen in degradation rate of the two disks: GDSC was almost completely degraded after 28 days, whereas HDSC remained intact. Degradation of GDSC occurred through collagenolytic activity and phagocytosis by macrophages. Phagocytosis was observed at day 2 and day 21. IL-13 was only observed in HDSC, and this resulted in the presence of giant cells in HDSC. These giant cells produced IL-10, that promoted TIMP-1 expression and that inhibits collagenolytic and phagocytic activity. We conclude that the function of macrophages in mice is largely influenced by differences in micro-environment induced by GDSC and HDSC and that the presence/absence of neutrophils play a major role in the shaping of this micro-environment. |
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Authors:
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Qingsong Ye; Martin C Harmsen; Marja J A van Luyn; Ruud A Bank |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-09 |
Journal Detail:
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Title: Biomaterials Volume: 31 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-10-18 Completed Date: 2011-01-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 9192-201 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Stem Cell & Tissue Engineering Research Group, Medical Biology Section, Department of Pathology & Medical Biology, University Medical Centre Groningen, University of Groningen, Hanzeplein 1, Groningen, The Netherlands. qingsongye@hotmail.com |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Collagen / chemistry* Cross-Linking Reagents / chemistry, pharmacology* Foreign-Body Reaction / chemically induced*, immunology, metabolism* Giant Cells / metabolism Glutaral / chemistry, pharmacology Immunohistochemistry Interferon-gamma / metabolism Interleukin-10 / metabolism Interleukin-13 / metabolism Isocyanates / chemistry, pharmacology Male Mice Mice, Inbred C57BL Neutrophil Infiltration / drug effects Neutrophils / drug effects, immunology* Phagocytosis / drug effects Sheep Tissue Inhibitor of Metalloproteinase-1 / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Cross-Linking Reagents; 0/Interleukin-13; 0/Isocyanates; 0/Tissue Inhibitor of Metalloproteinase-1; 111-30-8/Glutaral; 130068-27-8/Interleukin-10; 82115-62-6/Interferon-gamma; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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