Document Detail


The relationship between PTEN expression and anoikis in human lung carcinoma cell lines.
MedLine Citation:
PMID:  12098769     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The relationship between PTEN expression and anoikis in 8 different human lung carcinoma cell (HLCC) lines, and PKB and FAK effects in the process were studied. Northern blots and Western blots were carried out to analyze PTEN expression at mRNA and protein levels and the phosphorylation level of PKB /FAK in HLCC lines, and anoikis was examined by using DNA Ladder analysis. It was shown that PTEN mRNA expression was at similar level in 8 HLCC lines. But protein expression did not accord with it's mRNA expression. There were no PTEN protein detectable in 95C 95D and A(1) cells; very low PTEN protein was expressed in A(549) and A(4) A(7) cells; and high PTEN protein expressed in H(460) cells. There were no deletion and point mutation in 95D and H(460) cells; and mRNA stability in 95D cells was much lower than that of H(460) cells. Anoikis was observed in PTEN high-expressed H(460) cells with serum lack and loss of adhesion, and 10% serum prevented it from anoikis. PTEN decreased the phosphorylation level of PKB and down-regulated FAK expression in HLCC. These results suggest that PTEN expression is different in 8 HLCC lines, and PTEN was involved in anoikis.
Authors:
Qun-Ying Lei; Li-Ying Wang; Zhen-Yu Dai; Xi-Liang Zha
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica     Volume:  34     ISSN:  0582-9879     ISO Abbreviation:  Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-07-05     Completed Date:  2002-08-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  20730160R     Medline TA:  Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)     Country:  China    
Other Details:
Languages:  eng     Pagination:  463-8     Citation Subset:  IM    
Affiliation:
Key Laboratory of Glycoconjugate Research, Ministry of Health, Biochemistry Department, Fudan University Medical College, Shanghai 200032, China. xlzha@shmu.edu.cn
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MeSH Terms
Descriptor/Qualifier:
Anoikis / genetics*
Blotting, Northern
Blotting, Western
Gene Expression Regulation, Neoplastic
Humans
Lung Neoplasms / genetics*,  pathology
PTEN Phosphohydrolase
Phosphoric Monoester Hydrolases / genetics*,  metabolism
RNA Stability
RNA, Messenger / genetics,  metabolism
Tumor Cells, Cultured
Tumor Suppressor Proteins / genetics*,  metabolism
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Tumor Suppressor Proteins; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC 3.1.3.48/PTEN protein, human; EC 3.1.3.67/PTEN Phosphohydrolase

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