Document Detail


The regulatory role of sialic acids in the response of class II reactive T cell hybridomas to allogeneic B cells.
MedLine Citation:
PMID:  3531333     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two different kinds of alloreactive T cell hybridomas were established in previous experiments. One is reactive and the other is nonreactive to allogeneic I-A region-associated membrane antigen (mIa) on B cells. In the present experiments the difference between these hybridomas were analyzed by using representative clones, B cell mIa-reactive clone CB-11.4, and nonreactive clone HTB-9.3. Unresponsiveness of HTB-9.3 clone to allogeneic B cells could not be due to the inability of B cells in interleukin 1 production or the density of mIa molecules on B cells. HTB-9.3 clone could respond to C57BL/6 mouse B cells treated with neuraminidase (Nase), and Nase-treated HTB-9.3 clone could respond to normal B cells from C57BL/6 mouse, indicating that sialic acid on both B cells and HTB-9.3 clone plays a regulatory role in the alloreactivity of the clone. In response to B cells from C57BL/6 mouse, T cells from C3H/He mouse spleen showed similar reactivity to HTB-9.3 clone; that is, T cells could respond to Nase-treated B cells, and Nase-treated T cells to B cells, and T cells primed with C57BL/6 spleen cells in vitro showed similar reactivity to CB-11.4 clone. These results suggest that HTB-9.3 clone represents virgin T cells and CB-11.4 clone-primed T cells at least in alloreactivity. Anti-L3T4a was shown to block alloreactivities of both T cell hybridomas and splenic T cells against B cells more efficiently than against splenic adherent cells. These results suggest that L3T4a on T cell plays more important role in allogeneic response to B cells than to splenic adherent cells.
Authors:
S Taira; T Kakiuchi; M Minami; H Nariuchi; S Taiara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  137     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1986 Oct 
Date Detail:
Created Date:  1986-11-07     Completed Date:  1986-11-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2448-54     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
B-Lymphocytes / immunology*
Cell Adhesion
Cells, Cultured
Clone Cells
Fluorescent Antibody Technique
Hybridomas / immunology
Interleukin-1 / isolation & purification
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
Neuraminidase / pharmacology*
Sialic Acids / physiology*
T-Lymphocytes / classification,  immunology*
Chemical
Reg. No./Substance:
0/Interleukin-1; 0/Sialic Acids; EC 3.2.1.18/Neuraminidase
Comments/Corrections
Erratum In:
J Immunol 1986 Dec 15;137(12):4021
Note: Taiara S [corrected to Taira S]

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