| The regulatory role of sialic acids in the response of class II reactive T cell hybridomas to allogeneic B cells. | |
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MedLine Citation:
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PMID: 3531333 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two different kinds of alloreactive T cell hybridomas were established in previous experiments. One is reactive and the other is nonreactive to allogeneic I-A region-associated membrane antigen (mIa) on B cells. In the present experiments the difference between these hybridomas were analyzed by using representative clones, B cell mIa-reactive clone CB-11.4, and nonreactive clone HTB-9.3. Unresponsiveness of HTB-9.3 clone to allogeneic B cells could not be due to the inability of B cells in interleukin 1 production or the density of mIa molecules on B cells. HTB-9.3 clone could respond to C57BL/6 mouse B cells treated with neuraminidase (Nase), and Nase-treated HTB-9.3 clone could respond to normal B cells from C57BL/6 mouse, indicating that sialic acid on both B cells and HTB-9.3 clone plays a regulatory role in the alloreactivity of the clone. In response to B cells from C57BL/6 mouse, T cells from C3H/He mouse spleen showed similar reactivity to HTB-9.3 clone; that is, T cells could respond to Nase-treated B cells, and Nase-treated T cells to B cells, and T cells primed with C57BL/6 spleen cells in vitro showed similar reactivity to CB-11.4 clone. These results suggest that HTB-9.3 clone represents virgin T cells and CB-11.4 clone-primed T cells at least in alloreactivity. Anti-L3T4a was shown to block alloreactivities of both T cell hybridomas and splenic T cells against B cells more efficiently than against splenic adherent cells. These results suggest that L3T4a on T cell plays more important role in allogeneic response to B cells than to splenic adherent cells. |
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Authors:
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S Taira; T Kakiuchi; M Minami; H Nariuchi; S Taiara |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 137 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 1986 Oct |
Date Detail:
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Created Date: 1986-11-07 Completed Date: 1986-11-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2448-54 Citation Subset: AIM; IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals B-Lymphocytes / immunology* Cell Adhesion Cells, Cultured Clone Cells Fluorescent Antibody Technique Hybridomas / immunology Interleukin-1 / isolation & purification Mice Mice, Inbred C3H Mice, Inbred C57BL Neuraminidase / pharmacology* Sialic Acids / physiology* T-Lymphocytes / classification, immunology* |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-1; 0/Sialic Acids; EC 3.2.1.18/Neuraminidase |
| Comments/Corrections | |
Erratum In:
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J Immunol 1986 Dec 15;137(12):4021 Note: Taiara S [corrected to Taira S] |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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