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The regulatory network of cluster-root function and development in phosphate-deficient white lupin (Lupinus albus) identified by transcriptome sequencing.
MedLine Citation:
PMID:  24635386     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Lupinus albus serves as model plant for root-induced mobilization of sparingly soluble soil phosphates via the formation of cluster-roots (CR) that mediate secretion of protons, citrate, phenolics and acid phosphatases (APases). This study employed next generation sequencing to investigate the molecular mechanisms behind these complex adaptive responses at the transcriptome level. We compared different stages of CR development, including pre-emergent (PE), juvenile (JU) and the mature (MA) stages. The results confirmed that the primary metabolism underwent significant modifications during CR maturation, promoting the biosynthesis of organic acids, as had been deduced from physiological studies. Citrate catabolism was down-regulated, associated with citrate accumulation in MA clusters. Up-regulation of the phenylpropanoid pathway reflected the accumulation of phenolics. Specific transcript expression of ALMT and MATE transporter genes correlated with the exudation of citrate and flavonoids. The expression of transcripts related to nucleotide degradation and APases in MA clusters coincided with the re-mobilization and hydrolysis of organic phosphate resources. Most interestingly, hormone-related gene expression suggested a central role of ethylene during CR maturation. This was associated with the up-regulation of the iron (Fe)-deficiency regulated network that mediates ethylene-induced expression of Fe-deficiency responses in other species. Finally, transcripts related to abscisic and jasmonic acids were up-regulated in MA clusters, while auxin- and brassinosteroid-related genes and cytokinin receptors were most strongly expressed during CR initiation. Key regulations proposed by the RNA-seq data were confirmed by RT-qPCR and some physiological analyses. A model for the gene network regulating cluster-root development and function is presented.
Authors:
Zhengrui Wang; Daniel Straub; Huaiyu Yang; Angelika Kania; Jianbo Shen; Uwe Ludewig; Günter Neumann
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-3-17
Journal Detail:
Title:  Physiologia plantarum     Volume:  -     ISSN:  1399-3054     ISO Abbreviation:  Physiol Plant     Publication Date:  2014 Mar 
Date Detail:
Created Date:  2014-3-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1256322     Medline TA:  Physiol Plant     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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This article is protected by copyright. All rights reserved.
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