Document Detail

The regulation of triglyceride synthesis and fatty acid synthesis in rat epididymal adipose tissue. Effects of altered dietary and hormonal conditions.
MedLine Citation:
PMID:  4249859     Owner:  NLM     Status:  MEDLINE    
1. Epididymal adipose tissues obtained from rats that had been previously starved, starved and refed a high fat diet for 72h, starved and refed bread for 144h or fed a normal diet were incubated in the presence of insulin+glucose or insulin+glucose+acetate. 2. Measurements were made of the whole-tissue concentrations of hexose phosphates, triose phosphates, glycerol 1-phosphate, 3-phosphoglycerate, 6-phosphogluconate, adenine nucleotides, acid-soluble CoA, long-chain fatty acyl-CoA, malate and citrate after 1h of incubation. The release of lactate, pyruvate and glycerol into the incubation medium during this period was also determined. 3. The rates of metabolism of glucose in the hexose monophosphate pathway, the glycolytic pathway, the citric acid cycle and into glyceride glycerol, fatty acids and lactate+pyruvate were also determined over a 2h period in similarly treated tissues. The metabolism of acetate to CO(2) and fatty acids in the presence of glucose was also measured. 4. The activities of acetyl-CoA carboxylase, fatty acid synthetase and isocitrate dehydrogenase were determined in adipose tissues from starved, starved and fat-refed, and alloxan-diabetic animals and also in tissues from animals that had been starved and refed bread for up to 96h. Changes in these activities were compared with the ability of similar tissues to incorporate [(14)C]glucose into fatty acids in vitro. 5. The activities of acetyl-CoA carboxylase and fatty acid synthetase roughly paralleled the ability of tissues to incorporate glucose into fatty acids. 6. Rates of triglyceride synthesis and fatty acid synthesis could not be correlated with tissue concentrations of long-chain fatty acyl-CoA, citrate or glycerol 1-phosphate. In some cases changes in phosphofructokinase flux rates could be correlated with changes in citrate concentration. 7. The main lesion in fatty acid synthesis in tissues from starved, starved and fat-refed, and alloxan-diabetic rats appeared to reside at the level of pyruvate utilization and to be related to the rate of endogenous lipolysis. 8. It is suggested that pyruvate utilization by the tissue may be regulated by the metabolism of fatty acids within the tissue. The significance of this in directing glucose utilization away from fatty acid synthesis and into glyceride-glycerol synthesis is discussed.
E D Saggerson; A L Greenbaum
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  119     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1970 Sep 
Date Detail:
Created Date:  1971-02-09     Completed Date:  1971-02-09     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  221-42     Citation Subset:  IM    
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MeSH Terms
Acetates / metabolism
Adipose Tissue / metabolism*
Citric Acid Cycle
Diabetes Mellitus, Experimental / metabolism*
Dietary Fats
Epididymis / metabolism
Fatty Acids / biosynthesis*
Glucose / metabolism
Hexosephosphates / metabolism
Isocitrate Dehydrogenase / metabolism
Ligases / metabolism
Phosphofructokinase-1 / metabolism
Starvation / metabolism
Triglycerides / biosynthesis*
Trioses / metabolism
Reg. No./Substance:
0/Acetates; 0/Dietary Fats; 0/Fatty Acids; 0/Hexosephosphates; 0/Triglycerides; 0/Trioses; 50-99-7/Glucose; EC Dehydrogenase; EC; EC 6.-/Ligases

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