| The regulation of tendon stem cell differentiation by the alignment of nanofibers. | |
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MedLine Citation:
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PMID: 19995669 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tendon is a specific connective tissue composed of parallel collagen fibers. The effect of this tissue-specific matrix orientation on stem cell differentiation has not been investigated. This study aimed to determine the effects of nanotopography on the differentiation of human tendon stem/progenitor cells (hTSPCs) and develop a biomimetic scaffold for tendon tissue engineering. The immuno-phenotype of fetal hTSPCs was identified by flow cytometry. The multipotency of hTSPCs toward osteogenesis, adipogenesis, and chondrogenesis was confirmed. Then, the hTSPCs were seeded onto aligned or randomly-oriented poly (l-lactic acid) nanofibers. Scanning electron micrographs showed that hTSPCs were spindle-shaped and well orientated on the aligned nanofibers. The expression of tendon-specific genes was significantly higher in hTSPCs growing on aligned nanofibers than those on randomly-oriented nanofibers in both normal and osteogenic media. In addition, alkaline phosphatase activity and alizarin red staining showed that the randomly-oriented fibrous scaffold induced osteogenesis, while the aligned scaffold hindered the process. Moreover, aligned cells expressed significantly higher levels of integrin alpha1, alpha5 and beta1 subunits, and myosin II B. In in vivo experiments, the aligned nanofibers induced the formation of spindle-shaped cells and tendon-like tissue. In conclusion, the aligned electrospun nanofiber structure provides an instructive microenvironment for hTSPC differentiation and may lead to the development of desirable engineered tendons. |
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Authors:
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Zi Yin; Xiao Chen; Jia Lin Chen; Wei Liang Shen; Thi Minh Hieu Nguyen; Ling Gao; Hong Wei Ouyang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-07 |
Journal Detail:
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Title: Biomaterials Volume: 31 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-01-29 Completed Date: 2010-04-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 2163-75 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2009 Elsevier Ltd. All rights reserved. |
Affiliation:
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Center for Stem Cell and Tissue Engineering, Department of Sports Medicine, School of Medicine, Zhejiang University, and Department of Orthopedic Surgery, 2nd Affiliated Hospital, Hangzhou, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD / metabolism Biocompatible Materials / chemistry, metabolism Cell Differentiation / physiology* Cell Proliferation Cells, Cultured Female Humans Lactic Acid / chemistry, metabolism Materials Testing Mice Mice, Nude Microscopy, Electron, Scanning Multipotent Stem Cells / cytology, physiology Nanofibers / chemistry* Polymers / chemistry, metabolism Stress, Mechanical Tendons / cytology*, physiology Tissue Engineering* Tissue Scaffolds* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Biocompatible Materials; 0/Polymers; 26100-51-6/poly(lactic acid); 50-21-5/Lactic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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