Document Detail


The regulation of focal adhesion complex formation and salivary gland epithelial cell organization by nanofibrous PLGA scaffolds.
MedLine Citation:
PMID:  22285464     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nanofiber scaffolds have been useful for engineering tissues derived from mesenchymal cells, but few studies have investigated their applicability for epithelial cell-derived tissues. In this study, we generated nanofiber (250 nm) or microfiber (1200 nm) scaffolds via electrospinning from the polymer, poly-l-lactic-co-glycolic acid (PLGA). Cell-scaffold contacts were visualized using fluorescent immunocytochemistry and laser scanning confocal microscopy. Focal adhesion (FA) proteins, such as phosphorylated FAK (Tyr397), paxillin (Tyr118), talin and vinculin were localized to FA complexes in adult cells grown on planar surfaces but were reduced and diffusely localized in cells grown on nanofiber surfaces, similar to the pattern observed in adult mouse salivary gland tissues. Significant differences in epithelial cell morphology and cell clustering were also observed and quantified, using image segmentation and computational cell-graph analyses. No statistically significant differences in scaffold stiffness between planar PLGA film controls compared to nanofibers scaffolds were detected using nanoindentation with atomic force microscopy, indicating that scaffold topography rather than mechanical properties accounts for changes in cell attachments and cell structure. Finally, PLGA nanofiber scaffolds could support the spontaneous self-organization and branching of dissociated embryonic salivary gland cells. Nanofiber scaffolds may therefore have applicability in the future for engineering an artificial salivary gland.
Authors:
Sharon J Sequeira; David A Soscia; Basak Oztan; Aaron P Mosier; Riffard Jean-Gilles; Anand Gadre; Nathaniel C Cady; Bülent Yener; James Castracane; Melinda Larsen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-01-27
Journal Detail:
Title:  Biomaterials     Volume:  33     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-02-08     Completed Date:  2012-08-15     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  3175-86     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Adhesion / physiology
Cell Survival
Cells, Cultured
Epithelial Cells / cytology*,  physiology*
Focal Adhesions / physiology*
Lactic Acid / chemistry*
Mice
Nanotubes / chemistry*,  ultrastructure
Polyglycolic Acid / chemistry*
Salivary Glands / cytology*,  physiology
Tissue Engineering / instrumentation*
Tissue Scaffolds*
Grant Support
ID/Acronym/Agency:
C06 RR015464/RR/NCRR NIH HHS; C06 RR015464-01/RR/NCRR NIH HHS; F32 DE020980-01A1/DE/NIDCR NIH HHS; F32DE020980/DE/NIDCR NIH HHS; R01 DE019244/DE/NIDCR NIH HHS; R01 DE019244/DE/NIDCR NIH HHS; R01 DE019244-04/DE/NIDCR NIH HHS; R21 DE019197/DE/NIDCR NIH HHS; R21 DE019197/DE/NIDCR NIH HHS; R21 DE019197-01/DE/NIDCR NIH HHS; R21 DE019197-02S1/DE/NIDCR NIH HHS; RC1 DE020402/DE/NIDCR NIH HHS; RC1 DE020402/DE/NIDCR NIH HHS; RC1 DE020402-01/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 33X04XA5AT/Lactic Acid
Comments/Corrections

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