Document Detail

The regulation of fatty acid chain elongation in rat liver microsomes: role of fasting and CoASH.
MedLine Citation:
PMID:  7918589     Owner:  NLM     Status:  MEDLINE    
The elongation system for palmitic acid in rat liver microsomes was decreased to 1/3 by fasting, while the elongation of eicosapentaenoic acid was not sensitive to fasting. The rate of eicosapentaenoic acid elongation in the fed state was 50% higher than using palmitic acid as a substrate. The saturated and polyunsaturated fatty acyl-CoA substrates exhibited positive cooperativity on the rate-limiting condensing step in the elongation system, with a Hill constant of approx. 2. An inhibition by CoASH on the total elongation reaction as well as on the condensation step was demonstrated using acyl-CoA substrates, and followed a hyperbolic pattern. The concentrations giving a 50% inhibition (30-70 microM) were in the range found in rat hepatocyte cytosol, indicating that free CoASH has the potential to act as a physiological regulator.
W H Reichelt; H J Grav; E N Christiansen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1214     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1994 Sep 
Date Detail:
Created Date:  1994-10-25     Completed Date:  1994-10-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  109-14     Citation Subset:  IM    
Institute for Nutrition Research, University of Oslo, Norway.
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MeSH Terms
Coenzyme A / isolation & purification,  metabolism*,  pharmacology
Eicosapentaenoic Acid / metabolism
Fatty Acids / chemistry,  metabolism*
Microsomes, Liver / enzymology,  metabolism*
Palmitic Acid
Palmitic Acids / metabolism
Rats, Wistar
Reg. No./Substance:
0/Fatty Acids; 0/Palmitic Acids; 1553-41-9/Eicosapentaenoic Acid; 57-10-3/Palmitic Acid; 85-61-0/Coenzyme A

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