| The regulation of arachidonate lipoxygenase metabolite formation in cells derived from intrauterine tissues. | |
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MedLine Citation:
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PMID: 7784469 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Products of arachidonic acid (AA) metabolism via the lipoxygenase pathways may have key roles in the maintenance of pregnancy and the onset of labor. We have determined whether calcium ionophores can modulate the rate of biosynthesis within the uterus of five important arachidonate lipoxygenase metabolites, i.e. leukotriene B4 (LTB4), LTC4, 5-hydroxyeicosatetraenoic acid (5-HETE), 12-HETE, and 15-HETE. Amnion, chorion, and decidual cells were isolated, grown to confluence and incubated with ionomycin. The production of LTB4, LTC4, 5-HETE, 12-HETE, and 15-HETE was determined using specific radioimmunoassays. Cell-specific, concentration-related stimulatory actions of ionomycin on 5-HETE, 12-HETE, 15-HETE, and LTC4 but not LTB4 production were found. A23187 had effects similar to ionomycin. Hence elevation of intracellular calcium levels can result in enhanced intrauterine production of arachidonate lipoxygenase metabolites that may affect pregnancy outcome. |
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Authors:
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S S Edwin; D Thai; S LaMarche; D W Branch; M D Mitchell |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Prostaglandins, leukotrienes, and essential fatty acids Volume: 52 ISSN: 0952-3278 ISO Abbreviation: Prostaglandins Leukot. Essent. Fatty Acids Publication Date: 1995 Apr |
Date Detail:
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Created Date: 1995-07-14 Completed Date: 1995-07-14 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8802730 Medline TA: Prostaglandins Leukot Essent Fatty Acids Country: SCOTLAND |
Other Details:
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Languages: eng Pagination: 229-33 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, University of Utah School of Medicine, Salt Lake City 84132, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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12-Hydroxy-5,8,10,14-eicosatetraenoic Acid Amnion / metabolism Arachidonic Acid / metabolism Calcimycin / pharmacology Chorion / metabolism Decidua / metabolism Female Humans Hydroxyeicosatetraenoic Acids / biosynthesis* Ionomycin / pharmacology Leukotriene B4 / biosynthesis* Leukotriene C4 / biosynthesis* Lipoxygenase / metabolism Pregnancy Uterus / drug effects, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Hydroxyeicosatetraenoic Acids; 506-32-1/Arachidonic Acid; 52665-69-7/Calcimycin; 56092-81-0/Ionomycin; 59985-28-3/12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; 71160-24-2/Leukotriene B4; 72025-60-6/Leukotriene C4; 72255-35-7/5-hydroxy-6,8,11,14-eicosatetraenoic acid; 73945-47-8/15-hydroxy-5,8,11,13-eicosatetraenoic acid; EC 1.13.11.12/Lipoxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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