Document Detail

The regulation of actin polymerization in differentiating U937 cells correlates with increased membrane levels of the pertussis-toxin-sensitive G-protein Gi2.
MedLine Citation:
PMID:  1645523     Owner:  NLM     Status:  MEDLINE    
Undifferentiated U937 cells appear to lack a capacity of increase cellular F-actin. However, electropermeabilized cells gain the ability to respond in this way to a guanine nucleotide analogue, guanosine 5'-[gamma-thio]trisphosphate (GTP[S]) after 1 h of treatment with dibutyryl cyclic AMP (db-cAMP). The results reported here show that the levels of membrane association of the G-protein Gi2 alpha increase with a time course identical with that of the GTP[S]-sensitivity of electropermeabilized cells. These results suggest that Gi2 alpha may be involved in the signal-transduction pathway leading to actin polymerization in db-cAMP-differentiated U937 cells.
B Sheth; P Banks; D R Burton; P N Monk
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  275 ( Pt 3)     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1991 May 
Date Detail:
Created Date:  1991-07-03     Completed Date:  1991-07-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  809-11     Citation Subset:  IM    
Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, U.K.
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MeSH Terms
Actins / metabolism*
Blotting, Western
Bucladesine / pharmacology
Cell Differentiation
Cell Line
Cell Membrane / metabolism*
GTP-Binding Proteins / metabolism*
Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology
Monocytes / cytology,  drug effects,  metabolism*
Pertussis Toxin*
Polymers / metabolism
Virulence Factors, Bordetella / pharmacology*
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Actins; 0/Polymers; 0/Virulence Factors, Bordetella; 362-74-3/Bucladesine; 37589-80-3/Guanosine 5'-O-(3-Thiotriphosphate); EC Toxin; EC 3.6.1.-/GTP-Binding Proteins

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