Document Detail

The reductive acetyl coenzyme A pathway: sequence and heterologous expression of active methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase from Clostridium thermoaceticum.
MedLine Citation:
PMID:  7928975     Owner:  NLM     Status:  MEDLINE    
The methyltransferase (MeTr) from Clostridium thermoaceticum transfers the N5-methyl group of (6S)-methyltetrahydrofolate to the cobalt center of a corrinoid/iron-sulfur protein in the acetyl coenzyme A pathway. MeTr was purified to homogeneity and shown to lack metals. The acsE gene encoding MeTr was sequenced and actively expressed in Escherichia coli at a level of 9% of cell protein. Regions in the sequence of MeTr and the E. coli cobalamin-dependent methionine synthase were found to share significant homology, suggesting that they may represent tetrahydrofolate-binding domains.
D L Roberts; S Zhao; T Doukov; S W Ragsdale
Related Documents :
1575465 - Metabolic pathways in paracoccus denitrificans and closely related bacteria in relation...
3350005 - Purification, properties and complete amino acid sequence of the ferredoxin from a gree...
15766235 - Ferritins: dynamic management of biological iron and oxygen chemistry.
9244285 - Cloning and expression of the two genes coding for l-serine dehydratase from peptostrep...
16519685 - Mutational analysis of functional domains in mrs2p, the mitochondrial mg2+ channel prot...
15734055 - Virus-like particle expression and assembly in plants: hepatitis b and norwalk viruses.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  176     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1994-10-28     Completed Date:  1994-10-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6127-30     Citation Subset:  IM    
Department of Biochemistry, University of Nebraska, Lincoln 68583-0718.
Data Bank Information
Bank Name/Acc. No.:
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / genetics
Acetyl Coenzyme A / metabolism
Amino Acid Sequence
Bacterial Proteins / metabolism*
Base Sequence
Cloning, Molecular
Clostridium / enzymology,  genetics*
Genes, Bacterial
Iron-Sulfur Proteins / metabolism*
Metals / analysis
Methyltransferases / genetics*,  isolation & purification
Molecular Sequence Data
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Tetrahydrofolates / metabolism*
Grant Support
Reg. No./Substance:
0/Bacterial Proteins; 0/C/FE-SP protein, Moorella thermoacetica; 0/Iron-Sulfur Proteins; 0/Metals; 0/Tetrahydrofolates; 134-35-0/5-methyltetrahydrofolate; 72-89-9/Acetyl Coenzyme A; EC 2.1.1.-/Methyltransferases; EC S-Methyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The Calvin cycle enzyme phosphoglycerate kinase of Xanthobacter flavus required for autotrophic CO2 ...
Next Document:  Involvement of folic acid and methionine in the synthesis of certain membrane-associated nucleotide ...