| The reductive acetyl coenzyme A pathway: sequence and heterologous expression of active methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase from Clostridium thermoaceticum. | |
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MedLine Citation:
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PMID: 7928975 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The methyltransferase (MeTr) from Clostridium thermoaceticum transfers the N5-methyl group of (6S)-methyltetrahydrofolate to the cobalt center of a corrinoid/iron-sulfur protein in the acetyl coenzyme A pathway. MeTr was purified to homogeneity and shown to lack metals. The acsE gene encoding MeTr was sequenced and actively expressed in Escherichia coli at a level of 9% of cell protein. Regions in the sequence of MeTr and the E. coli cobalamin-dependent methionine synthase were found to share significant homology, suggesting that they may represent tetrahydrofolate-binding domains. |
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Authors:
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D L Roberts; S Zhao; T Doukov; S W Ragsdale |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of bacteriology Volume: 176 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1994 Oct |
Date Detail:
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Created Date: 1994-10-28 Completed Date: 1994-10-28 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 6127-30 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University of Nebraska, Lincoln 68583-0718. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/L34780 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase
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genetics Acetyl Coenzyme A / metabolism Amino Acid Sequence Bacterial Proteins / metabolism* Base Sequence Cloning, Molecular Clostridium / enzymology, genetics* Genes, Bacterial Iron-Sulfur Proteins / metabolism* Metals / analysis Methyltransferases / genetics*, isolation & purification Molecular Sequence Data Sequence Analysis, DNA Sequence Homology, Amino Acid Tetrahydrofolates / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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GM39451/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/C/FE-SP protein, Moorella thermoacetica; 0/Iron-Sulfur Proteins; 0/Metals; 0/Tetrahydrofolates; 134-35-0/5-methyltetrahydrofolate; 72-89-9/Acetyl Coenzyme A; EC 2.1.1.-/Methyltransferases; EC 2.1.1.13/5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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