Document Detail


The recruitment of activation induced cytidine deaminase to the immunoglobulin locus by a regulatory element.
MedLine Citation:
PMID:  20334924     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Activation induced cytidine deaminase (AID) is critical for the diversification of immunoglobulin (Ig). AID is generally thought to function by deaminating cytidines into uridines in the target DNA within the Ig loci, and the subsequent processing of the uridines, through DNA repair or replication, could lead to three different forms of Ig diversification events: class switch recombination, somatic hypermutation and gene conversion. Although AID is important for effective immunity, its mutagenic activity needs to be restricted to the Ig loci in order to avoid rampant mutations in the genome. In our previous studies, we have identified an Ig lambda regulatory element (Region A) that is important for AID mediated gene conversion in chicken B cells, but its mechanism of function was unclear. In this report, we provide evidence that the regulatory element plays a role in recruiting AID to the Ig lambda locus.
Authors:
Yonghwan Kim; Ming Tian
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-23
Journal Detail:
Title:  Molecular immunology     Volume:  47     ISSN:  1872-9142     ISO Abbreviation:  Mol. Immunol.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-27     Completed Date:  2010-06-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905289     Medline TA:  Mol Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1860-5     Citation Subset:  IM    
Copyright Information:
(c) 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Section of Molecular Genetics and Microbiology, University of Texas at Austin, 1 University Station, A5000, Austin, TX 78712, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibody Diversity / genetics
Cell Line, Tumor
Chickens
Cytidine Deaminase / genetics,  metabolism*
Gene Rearrangement, B-Lymphocyte / genetics
Immunoglobulin lambda-Chains / genetics
Immunoglobulins / genetics*
Protein Binding
Regulatory Sequences, Nucleic Acid / genetics*
Sequence Deletion
Chemical
Reg. No./Substance:
0/Immunoglobulin lambda-Chains; 0/Immunoglobulins; EC 3.5.4.-/AICDA (activation-induced cytidine deaminase); EC 3.5.4.5/Cytidine Deaminase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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