Document Detail


A recombined protein (rSj16) derived from Schistosoma japonicum induces cell cycle arrest and apoptosis of murine myeloid leukemia cells.
MedLine Citation:
PMID:  23319265     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
rSj16, a recombined protein from Schistosoma japonicum, has been identified as an anti-inflammatory molecule. In this study, we demonstrated that rSj16 strongly suppressed the growth of murine myeloid leukemia WEHI-3B JCS cells in a dose- and time-dependent manner. rSj16 induced apoptosis by increasing the proportion of sub-G1 apoptotic cells as well as causing cell cycle arrest at the G0/G1 phase. The expressions of cyclin D1, D2, D3, and E, and Cdk 2, 4, and 6 genes in WEHI-3B JCS cells were significantly down-regulated at 24 h as measured by real-time PCR. Furthermore, apoptosis induced by rSj16 was confirmed by 4,6-diamidino-2-phenylindole nuclear staining assay and annexin V/propidium iodide double staining. A reduction of the mitochondrial membrane potential indicated an active involvement of mitochondria in the apoptosis process. rSj16 treatment induced an increase in the activity of caspase 3, 6, and 9, and expression of pro-apoptotic Bax. Meanwhile, the decreased expression of anti-apoptotic Bcl-2 was observed after rSj16 treatment. Taken together, our results implied that rSj16 can inhibit proliferation by inducing G0/G1 cell cycle arrest and apoptosis of murine myeloid leukemia cells via activation of the caspase-mediated mechanism by regulating the expression of Bcl-2 family.
Authors:
Fan Yang; Xi Sun; Jia Shen; Li-Ping Yu; Jin-Yi Liang; Huan-Qin Zheng; Zhong-Dao Wu
Related Documents :
23645725 - Premalignant lesions skew spleen cell responses to immune modulation by adipocytes.
24049665 - Perioperative dynamics of tlr2, tlr4, and trem-1 expression in monocyte subpopulations ...
23961055 - The role of caspase activation and mitochondrial depolarisation in cultured human apopt...
23954445 - Simultaneous knock-down of bcl-xl and mcl-1 induces apoptosis through bax activation in...
9203965 - Il-13 production by allergen-stimulated t cells is increased in allergic disease and as...
12537795 - New insights into maternal-fetal interactions at implantation.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-15
Journal Detail:
Title:  Parasitology research     Volume:  -     ISSN:  1432-1955     ISO Abbreviation:  Parasitol. Res.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8703571     Medline TA:  Parasitol Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, Guangdong, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A biomimetic chitosan composite with improved mechanical properties in wet conditions.
Next Document:  Acanthamoeba castellanii cysts: new ultrastructural findings.