Document Detail


A recombinant peroxisome proliferator response element-driven luciferase assay for evaluation of potential environmental obesogens.
MedLine Citation:
PMID:  20665227     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
A recombinant Huh7-PPRE-Luc cell line for analyzing the peroxisome proliferator response element (PPRE)-driven luciferase activity was established. The cells exhibited a good dose-response induction in PPRE-driven luciferase activity by three subtypes of peroxisome proliferator-activated receptor (PPAR) agonists as well as by a retinoid X receptor agonist, 9-cis-retinoic acid. Among five environmental chemicals tested, benzyl butyl phthalate and bisphenol induced PPRE-driven luciferase activation in Huh7-PPRE-Luc cells and caused adipogenic differentiation of 3T3-L1 cells. This recombinant Huh7-PPRE-Luc cell line would be useful for screening potential environmental obesogens with PPAR activity.
Authors:
Ya-Fen Wang; How-Ran Chao; Chia-Hsin Wu; Chu-Han Tseng; Ya-Ting Kuo; Tsui-Chun Tsou
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-28
Journal Detail:
Title:  Biotechnology letters     Volume:  32     ISSN:  1573-6776     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1789-96     Citation Subset:  IM    
Affiliation:
Department of Bioenvironmental Engineering, Chung Yuan Christian University, Chungli, 320, Taiwan.
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