| A recombinant peroxisome proliferator response element-driven luciferase assay for evaluation of potential environmental obesogens. | |
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MedLine Citation:
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PMID: 20665227 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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A recombinant Huh7-PPRE-Luc cell line for analyzing the peroxisome proliferator response element (PPRE)-driven luciferase activity was established. The cells exhibited a good dose-response induction in PPRE-driven luciferase activity by three subtypes of peroxisome proliferator-activated receptor (PPAR) agonists as well as by a retinoid X receptor agonist, 9-cis-retinoic acid. Among five environmental chemicals tested, benzyl butyl phthalate and bisphenol induced PPRE-driven luciferase activation in Huh7-PPRE-Luc cells and caused adipogenic differentiation of 3T3-L1 cells. This recombinant Huh7-PPRE-Luc cell line would be useful for screening potential environmental obesogens with PPAR activity. |
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Authors:
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Ya-Fen Wang; How-Ran Chao; Chia-Hsin Wu; Chu-Han Tseng; Ya-Ting Kuo; Tsui-Chun Tsou |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-28 |
Journal Detail:
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Title: Biotechnology letters Volume: 32 ISSN: 1573-6776 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-05 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1789-96 Citation Subset: IM |
Affiliation:
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Department of Bioenvironmental Engineering, Chung Yuan Christian University, Chungli, 320, Taiwan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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