| A rearranged EP300 gene in the human B-cell lymphoma cell line RC-K8 encodes a disabled transcriptional co-activator that contributes to cell growth and oncogenicity. | |
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MedLine Citation:
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PMID: 21232847 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human diffuse large B-cell lymphoma cell line RC-K8 has an altered EP300 locus that encodes a C-terminally truncated histone acetyltransferase (HAT) protein (p300ΔC). We now show that p300ΔC contains 1047N-terminal amino acids of p300 fused to 25 amino acids encoded by sequences from chromosome 6. Over-expressed p300ΔC localized to nuclear subdomains and interacted with transcription factor REL. p300ΔC did not function as a co-activator for REL-directed transactivation, and blocked the ability of wild-type p300 to enhance transcriptional activation by REL. Knock down of p300ΔC in RC-K8 cells reduced their growth in both liquid culture and soft agar. Truncations of p300 were not found in eight other B-lymphoma cell lines. These results suggest that p300ΔC contributes to the oncogenic state of RC-K8 cells by acting as a defective co-activator. |
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Authors:
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Michael R Garbati; Ryan C Thompson; Leila Haery; Thomas D Gilmore |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-01-12 |
Journal Detail:
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Title: Cancer letters Volume: 302 ISSN: 1872-7980 ISO Abbreviation: Cancer Lett. Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-02-02 Completed Date: 2011-04-22 Revised Date: 2012-09-20 |
Medline Journal Info:
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Nlm Unique ID: 7600053 Medline TA: Cancer Lett Country: Ireland |
Other Details:
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Languages: eng Pagination: 76-83 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Department of Biology, Boston University, Boston, MA 02215, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Blotting, Western Cell Line Cell Line, Tumor Cell Proliferation* E1A-Associated p300 Protein / genetics*, metabolism Fluorescent Antibody Technique, Indirect Gene Rearrangement* HEK293 Cells Histone Acetyltransferases / genetics*, metabolism Humans Luciferases / genetics, metabolism Lymphoma, Large B-Cell, Diffuse / genetics, metabolism, pathology Molecular Sequence Data Protein Binding Proto-Oncogene Proteins c-rel / genetics, metabolism RNA Interference Transcriptional Activation Transfection |
| Grant Support | |
ID/Acronym/Agency:
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CA047763/CA/NCI NIH HHS; CA047763-21S3/CA/NCI NIH HHS; R01 CA047763-20S1/CA/NCI NIH HHS; R01 CA047763-21/CA/NCI NIH HHS; T32 HL007501/HL/NHLBI NIH HHS; T32 HL007501-30/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Proto-Oncogene Proteins c-rel; EC 1.13.12.-/Luciferases; EC 2.3.1.48/E1A-Associated p300 Protein; EC 2.3.1.48/EP300 protein, human; EC 2.3.1.48/Histone Acetyltransferases |
| Comments/Corrections | |
Erratum In:
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Cancer Lett. 2012 Feb 1;315(1):96 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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