Document Detail


A rearranged EP300 gene in the human B-cell lymphoma cell line RC-K8 encodes a disabled transcriptional co-activator that contributes to cell growth and oncogenicity.
MedLine Citation:
PMID:  21232847     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human diffuse large B-cell lymphoma cell line RC-K8 has an altered EP300 locus that encodes a C-terminally truncated histone acetyltransferase (HAT) protein (p300ΔC). We now show that p300ΔC contains 1047N-terminal amino acids of p300 fused to 25 amino acids encoded by sequences from chromosome 6. Over-expressed p300ΔC localized to nuclear subdomains and interacted with transcription factor REL. p300ΔC did not function as a co-activator for REL-directed transactivation, and blocked the ability of wild-type p300 to enhance transcriptional activation by REL. Knock down of p300ΔC in RC-K8 cells reduced their growth in both liquid culture and soft agar. Truncations of p300 were not found in eight other B-lymphoma cell lines. These results suggest that p300ΔC contributes to the oncogenic state of RC-K8 cells by acting as a defective co-activator.
Authors:
Michael R Garbati; Ryan C Thompson; Leila Haery; Thomas D Gilmore
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-01-12
Journal Detail:
Title:  Cancer letters     Volume:  302     ISSN:  1872-7980     ISO Abbreviation:  Cancer Lett.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-02     Completed Date:  2011-04-22     Revised Date:  2013-07-02    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  76-83     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Department of Biology, Boston University, Boston, MA 02215, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Blotting, Western
Cell Line
Cell Line, Tumor
Cell Proliferation*
E1A-Associated p300 Protein / genetics*,  metabolism
Fluorescent Antibody Technique, Indirect
Gene Rearrangement*
HEK293 Cells
Histone Acetyltransferases / genetics*,  metabolism
Humans
Luciferases / genetics,  metabolism
Lymphoma, Large B-Cell, Diffuse / genetics,  metabolism,  pathology
Molecular Sequence Data
Protein Binding
Proto-Oncogene Proteins c-rel / genetics,  metabolism
RNA Interference
Transcriptional Activation
Transfection
Grant Support
ID/Acronym/Agency:
CA047763/CA/NCI NIH HHS; CA047763-21S3/CA/NCI NIH HHS; R01 CA047763-20S1/CA/NCI NIH HHS; R01 CA047763-21/CA/NCI NIH HHS; T32 HL007501/HL/NHLBI NIH HHS; T32 HL007501-30/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins c-rel; EC 1.13.12.-/Luciferases; EC 2.3.1.48/E1A-Associated p300 Protein; EC 2.3.1.48/EP300 protein, human; EC 2.3.1.48/Histone Acetyltransferases
Comments/Corrections
Erratum In:
Cancer Lett. 2012 Feb 1;315(1):96

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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