| A real-time assay for monitoring nucleic acid cleavage by quadruplex formation. | |
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MedLine Citation:
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PMID: 17071965 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Direct and straightforward methods to follow nucleic acid cleavage are needed. A spectrophotometric quadruplex formation assay (QFA) was developed, which allows real-time monitoring of site-specific cleavage of nucleic acids. QFA was applied to study both protein and nucleic acid restriction enzymes, and was demonstrated to accurately determine Michaelis-Menten parameters for the cleavage reaction catalyzed by EcoRI. QFA can be used to study the mechanisms of protein-nucleic acid recognition. QFA is also a useful tool for dissecting individual nicking rates of a double-stranded cleavage. |
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Authors:
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Besik I Kankia |
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Publication Detail:
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Type: Evaluation Studies; Journal Article Date: 2006-10-28 |
Journal Detail:
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Title: Nucleic acids research Volume: 34 ISSN: 1362-4962 ISO Abbreviation: Nucleic Acids Res. Publication Date: 2006 |
Date Detail:
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Created Date: 2006-11-30 Completed Date: 2006-12-20 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: England |
Other Details:
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Languages: eng Pagination: e141 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University of Minnesota, MN 55455, USA. bkankia@umn.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aptamers, Nucleotide
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analysis Circular Dichroism DNA / analysis, metabolism* DNA Restriction Enzymes / metabolism DNA, Catalytic / metabolism Deoxyribonuclease EcoRI / metabolism G-Quadruplexes Kinetics RNA / metabolism* Spectrophotometry, Ultraviolet / methods* |
| Chemical | |
Reg. No./Substance:
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0/Aptamers, Nucleotide; 0/DNA, Catalytic; 145563-68-4/thrombin aptamer; 63231-63-0/RNA; 9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes; EC 3.1.21.-/Deoxyribonuclease EcoRI |
| Comments/Corrections | |
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