Document Detail


The reaction of 2,4,6-trinitrobenzenesulphonic acid with amino acids, Peptides and proteins.
MedLine Citation:
PMID:  5667253     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. The kinetics of the reaction of 2,4,6-trinitrobenzenesulphonic acid with various amino acids, peptides and proteins were studied by spectrophotometry. 2. The reaction of the alpha- and in-amino groups in simple amino acids was found to be second-order, and the unprotonated amino group was shown to be the reactive species. 3. By allowing for the concentration of unreactive -NH(3) (+) group, intrinsic reactivities for the free amino groups were derived and shown to be correlated with the basicities. 4. The SH group of N-acetylcysteine was found to be more reactive to 2,4,6-trinitrobenzenesulphonic acid than most amino groups. 5. The reactions of insulin, chymotrypsinogen and ribonuclease with 2,4,6-trinitrobenzenesulphonic acid were analysed in terms of three exponential rate curves, each referring to one or more amino groups of the proteins. 6. The reaction of lysozyme with 2,4,6-trinitrobenzenesulphonic acid was found to display an acceleration effect. 7. From the reaction of 2,4,6-trinitrobenzenesulphonic acid with glutamate dehydrogenase at several enzyme concentrations, it was possible to discern two sets of amino groups of different reactivity, and to show that the number of groups in each set was decreased by aggregation of the enzyme.
Authors:
R B Freedman; G K Radda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  108     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1968 Jul 
Date Detail:
Created Date:  1968-10-02     Completed Date:  1968-10-02     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  383-91     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids*
Chemical Phenomena
Chemistry
Chymotrypsin
Enzyme Precursors
Glutamate Dehydrogenase
Hydrogen-Ion Concentration
Insulin
Kinetics
Muramidase
Peptides*
Proteins*
Ribonucleases
Spectrophotometry
Sulfonic Acids*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Enzyme Precursors; 0/Peptides; 0/Proteins; 0/Sulfonic Acids; 11061-68-0/Insulin; EC 1.4.1.2/Glutamate Dehydrogenase; EC 3.1.-/Ribonucleases; EC 3.2.1.17/Muramidase; EC 3.4.21.1/Chymotrypsin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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