| The reaction of 2,4,6-trinitrobenzenesulphonic acid with amino acids, Peptides and proteins. | |
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MedLine Citation:
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PMID: 5667253 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. The kinetics of the reaction of 2,4,6-trinitrobenzenesulphonic acid with various amino acids, peptides and proteins were studied by spectrophotometry. 2. The reaction of the alpha- and in-amino groups in simple amino acids was found to be second-order, and the unprotonated amino group was shown to be the reactive species. 3. By allowing for the concentration of unreactive -NH(3) (+) group, intrinsic reactivities for the free amino groups were derived and shown to be correlated with the basicities. 4. The SH group of N-acetylcysteine was found to be more reactive to 2,4,6-trinitrobenzenesulphonic acid than most amino groups. 5. The reactions of insulin, chymotrypsinogen and ribonuclease with 2,4,6-trinitrobenzenesulphonic acid were analysed in terms of three exponential rate curves, each referring to one or more amino groups of the proteins. 6. The reaction of lysozyme with 2,4,6-trinitrobenzenesulphonic acid was found to display an acceleration effect. 7. From the reaction of 2,4,6-trinitrobenzenesulphonic acid with glutamate dehydrogenase at several enzyme concentrations, it was possible to discern two sets of amino groups of different reactivity, and to show that the number of groups in each set was decreased by aggregation of the enzyme. |
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Authors:
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R B Freedman; G K Radda |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Biochemical journal Volume: 108 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1968 Jul |
Date Detail:
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Created Date: 1968-10-02 Completed Date: 1968-10-02 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 383-91 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids* Chemical Phenomena Chemistry Chymotrypsin Enzyme Precursors Glutamate Dehydrogenase Hydrogen-Ion Concentration Insulin Kinetics Muramidase Peptides* Proteins* Ribonucleases Spectrophotometry Sulfonic Acids* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Enzyme Precursors; 0/Peptides; 0/Proteins; 0/Sulfonic Acids; 11061-68-0/Insulin; EC 1.4.1.2/Glutamate Dehydrogenase; EC 3.1.-/Ribonucleases; EC 3.2.1.17/Muramidase; EC 3.4.21.1/Chymotrypsin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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