Document Detail


A rational approach to oocyte cryopreservation.
MedLine Citation:
PMID:  15949213     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reports of clinical pregnancies from cryopreserved human oocytes have been steadily increasing in recent years. However, success in terms of births per thawed oocyte remains poor. A wide variety of freezing techniques has been used lately, but modifications to protocols are made on an empirical basis. Methods of cryopreservation are often poorly described or protocols are not strictly adhered to, resulting in variability of outcome. The first stage of a freezing protocol is exposure to cryoprotectant. If performed inappropriately, such exposure can result in damage due to chemical toxicity and/or osmotic stress. Measurement of cell volume change during exposure to cryoprotectants demonstrates the extent of osmotic stress experienced by that cell. Such measurements have been performed during perfusion of murine and human oocytes with cryoprotectant concentrations commonly used for cryopreservation of these cells. It has been demonstrated that changes in the cryoprotectant type, concentration and temperature of exposure can dramatically affect the extent of cell volume change. Even small changes in duration of exposure to cryoprotectant prior to cooling can result in drastic changes in cellular hydration. Such factors will potentially influence the ability of the cell to survive the stresses experienced during the subsequent stages of the cryopreservation protocol.
Authors:
S J Paynter
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  10     ISSN:  1472-6483     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-06-13     Completed Date:  2005-08-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  578-86     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynaecology, Wales College of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK. Paynter@cardiff.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Membrane Permeability
Cryopreservation / methods*
Cryoprotective Agents / pharmacology
Dose-Response Relationship, Drug
Female
Humans
Mice
Mice, Inbred Strains
Oocytes / drug effects,  physiology*
Propylene Glycol / pharmacology
Sucrose / pharmacology
Temperature
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 57-50-1/Sucrose; 57-55-6/Propylene Glycol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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