| The rat whole embryo culture assay using the dysmorphology score system. | |
| | |
MedLine Citation:
|
PMID: 23138920 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
The rat whole embryo culture (WEC) system has been used extensively for characterizing teratogenic properties of test chemicals. In this chapter, we describe the methodology for culturing rat embryos as well as a new morphological score system, the Dysmorphology Score (DMS) system for assessing morphology of mid gestation (gestational day 11) rat embryos. In contrast to the developmental stage focused scoring associated with the Brown and Fabro score system, this new score system assesses the respective degree of severity of dysmorphology, which delineates normal from abnormal morphology of specific embryonic structures and organ systems. This score system generates an approach that allows rapid identification and quantification of adverse developmental findings, making it conducive for characterization of compounds for teratogenic properties and screening activities. |
| | |
Authors:
|
Cindy Zhang; Julie Panzica-Kelly; Karen Augustine-Rauch |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Methods in molecular biology (Clifton, N.J.) Volume: 947 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
|
Created Date: 2012-11-09 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 423-50 Citation Subset: IM |
Affiliation:
|
Bristol Myers Squibb Co, Pennington, NJ, USA, cindy.zhang@bms.com. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Frog embryo teratogenesis assay on Xenopus and predictivity compared with in vivo Mammalian studies.
Next Document: Toxicogenomic approaches in developmental toxicology testing.