Document Detail

A rat model of monitoring liver allograft rejection.
MedLine Citation:
PMID:  9089993     Owner:  NLM     Status:  MEDLINE    
Rat models are often used to study liver allograft rejection. We have established a model for rat liver allograft rejection, monitored by fine needle aspiration biopsy (FNAB), in the strain combination PVG-to-BN with a mean survival time of 37 +/- 20 days. In this model, we observed acute rejection with an intense peak of lymphoid blasts and lymphocyte-dominated inflammation in the FNAB [9.1 +/- 3.0 corrected increment units (CIU)], and an eventual increase in macrophages (up to 4.2 +/- 4.4 CIU), together with fibrosis and parenchymal necrosis in the graft. Markers of immune activation, such as an increase in IL-2-receptor (from 1% +/- 2% to 21% +/- 13%) and class II (from 20% +/- 9% to 43% +/- 13%) expressing lymphoid cells and induction of ICAM-1 in the graft, were consistent with the overall cellular response. The FNAB correlated well with parallel graft histology. In this rat model, the atraumatic monitoring makes a close follow-up possible without having to sacrifice the experimental animals. This saves work, animals, and costs in the study of liver rejection.
T Martelius; H Mäkisalo; K Höckerstedt; E Taskinen; I Lautenschlager
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Transplant international : official journal of the European Society for Organ Transplantation     Volume:  10     ISSN:  0934-0874     ISO Abbreviation:  Transpl. Int.     Publication Date:  1997  
Date Detail:
Created Date:  1997-06-09     Completed Date:  1997-06-09     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8908516     Medline TA:  Transpl Int     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  103-8     Citation Subset:  IM    
Fourth Department of Surgery, Helsinki University Central Hospital, Finland.
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MeSH Terms
Bile Ducts / pathology
Biological Markers
Biopsy, Needle
Graft Rejection / immunology,  pathology*
Graft Survival
Histocompatibility Antigens Class II / biosynthesis
Intercellular Adhesion Molecule-1 / biosynthesis
Liver Cirrhosis, Experimental / pathology
Liver Transplantation / immunology,  pathology*
Rats, Inbred BN
Rats, Inbred Strains
Receptors, Interleukin-2 / biosynthesis
Time Factors
Transplantation, Homologous
Reg. No./Substance:
0/Biological Markers; 0/Histocompatibility Antigens Class II; 0/Receptors, Interleukin-2; 126547-89-5/Intercellular Adhesion Molecule-1

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