| A rat model of exercise-induced asthma: a nonspecific response to a specific immunogen. | |
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MedLine Citation:
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PMID: 21228339 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Exercise-induced bronchoconstriction (EIB) is common; however, key aspects of its pathogenesis are still unclear. We investigated the feasibility of adapting an established animal model of asthma to investigate the earliest stages of EIB. The hypothesis was that a single exposure to a normally innocuous, and brief, exercise challenge could trigger EIB symptoms in rats previously sensitized to ovalbumin (OVA) but otherwise unchallenged. Brown-Norway rats were sensitized by intraperitoneal injection of OVA at 0 and 2 wk. At week 3, animals were exposed to either aerosolized OVA (SS) or exercise (EXS). A trained, blinded, clinical observer graded EIB by respiratory sounds. Plasma and lung cytokine levels were analyzed. No control rats with or without exercise (EX, CON) showed evidence of EIB. Eighty percent of the SS group demonstrated abnormal breath sounds upon exposure to aerosolized OVA. Approximately 30% of EXS rats sensitized to OVA but exposed only to exercise had abnormal breath sounds. Lung tissue levels of TNF-α, IL-1α, growth-related oncogene/keratinocyte/chemoattractant, and IFN-γ were significantly higher (P < 0.001) in the SS group, relative to all other groups. Changes in most of these cytokines were not notable in the EXS rats, suggesting a different mechanism of EIB. Remarkably, IFN-γ, but not the other cytokines measured, was significantly elevated following brief exercise in both sensitized and unsensitized rats. Exercise led to detectable breathing sound abnormalities in sensitized rats, but less severe than those observed following classical OVA challenge. Precisely how this immune crossover occurs is not known, but this model may be useful in elucidating essential mechanisms of EIB. |
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Authors:
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Einat Kodesh; Frank Zaldivar; Christina Schwindt; Phuc Tran; Alvin Yu; Marinelle Camilon; Dwight M Nance; Szu-Yun Leu; Dan Cooper; Gregory R Adams |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-01-12 |
Journal Detail:
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Title: American journal of physiology. Regulatory, integrative and comparative physiology Volume: 300 ISSN: 1522-1490 ISO Abbreviation: Am. J. Physiol. Regul. Integr. Comp. Physiol. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-04-04 Completed Date: 2011-06-16 Revised Date: 2012-04-02 |
Medline Journal Info:
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Nlm Unique ID: 100901230 Medline TA: Am J Physiol Regul Integr Comp Physiol Country: United States |
Other Details:
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Languages: eng Pagination: R917-24 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, University of California, Irvine, Irvine, California 92697-4560, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Asthma / chemically induced, pathology*, physiopathology* Bronchoconstriction / physiology* Cytokines / blood, metabolism Disease Models, Animal Histamine / metabolism Immunoglobulin E / metabolism Lung / metabolism, physiopathology Male Neutrophils / pathology Ovalbumin / adverse effects Physical Conditioning, Animal / physiology* Rats Rats, Inbred BN Respiratory Sounds / physiology |
| Grant Support | |
ID/Acronym/Agency:
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P01HD048721/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 37341-29-0/Immunoglobulin E; 51-45-6/Histamine; 9006-59-1/Ovalbumin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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