| Is rat liver affected by non-alcoholic steatosis more susceptible to the acute toxic effect of thioacetamide? | |
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MedLine Citation:
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PMID: 21410800 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Non-alcoholic fatty liver disease (NAFLD) is the most common chronic condition of the liver in the western world. There is only little evidence about altered sensitivity of steatotic liver to acute toxic injury. The aim of this project was to test whether hepatic steatosis sensitizes rat liver to acute toxic injury induced by thioacetamide (TAA). Male Sprague-Dawley rats were fed ad libitum a standard pelleted diet (ST-1, 10% energy fat) and high-fat gelled diet (HFGD, 71% energy fat) for 6 weeks and then TAA was applied intraperitoneally in one dose of 100 mg/kg. Animals were sacrificed in 24-, 48- and 72-h interval after TAA administration. We assessed the serum biochemistry, the hepatic reduced glutathione, thiobarbituric acid reactive substances, cytokine concentration, the respiration of isolated liver mitochondria and histopathological samples (H+E, Sudan III, bromodeoxyuridine [BrdU] incorporation). Activities of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase and concentration of serum bilirubin were significantly higher in HFGD groups after application of TAA, compared to ST-1. There were no differences in activities of respiratory complexes I and II. Serum tumour necrosis factor alpha at 24 and 48 h, liver tissue interleukin-6 at 72 h and transforming growth factor β1 at 24 and 48 h were elevated in TAA-administrated rats fed with HFGD, but not ST-1. TAA-induced centrilobular necrosis and subsequent regenerative response of the liver were higher in HFGD-fed rats in comparison with ST-1. Liver affected by NAFLD, compared to non-steatotic liver, is more sensitive to toxic effect of TAA. |
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Authors:
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Otto Kučera; Halka Lotková; Pavla Staňková; Miroslav Podhola; Tomáš Roušar; Vojtěch Mezera; Zuzana Cervinková |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2011-03-17 |
Journal Detail:
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Title: International journal of experimental pathology Volume: 92 ISSN: 1365-2613 ISO Abbreviation: Int J Exp Pathol Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-07-04 Completed Date: 2011-09-08 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 9014042 Medline TA: Int J Exp Pathol Country: England |
Other Details:
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Languages: eng Pagination: 281-9 Citation Subset: IM |
Copyright Information:
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© 2011 The Authors. International Journal of Experimental Pathology © 2011 International Journal of Experimental Pathology. |
Affiliation:
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Department of Physiology, Faculty of Medicine in Hradec Králové, Charles University in Prague, Hradec Králové, Czech Republic. kucerao@lfhk.cuni.cz |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carcinogens / toxicity* Cell Proliferation / drug effects Cholesterol / metabolism Cytokines / blood Dietary Fats / adverse effects Disease Models, Animal Electron Transport Complex I / drug effects, physiology Electron Transport Complex II / drug effects, physiology Fatty Liver / blood, chemically induced, pathology* Liver / drug effects*, metabolism, pathology* Male Rats Rats, Sprague-Dawley Thioacetamide / toxicity* Thiobarbituric Acid Reactive Substances / metabolism Triglycerides / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Carcinogens; 0/Cytokines; 0/Dietary Fats; 0/Thiobarbituric Acid Reactive Substances; 0/Triglycerides; 57-88-5/Cholesterol; 62-55-5/Thioacetamide; EC 1.3.5.1/Electron Transport Complex II; EC 1.6.5.3/Electron Transport Complex I; EC 1.6.5.3/respiratory complex II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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