| The rat hepatoma cell line H4-II-E-C3 expresses high activities of the high-affinity glutamate transporter GLT-1A. | |
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MedLine Citation:
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PMID: 11068035 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The rate of uptake of aspartate into the rat hepatoma cell line H4-II-E-C3 is very much higher than that exhibited by normal rat hepatocytes. Using an RT-PCR-based strategy, a glutamate transporter resembling mouse liver GLT-1A has been cloned from H4-II-E-C3 cells. Northern blotting confirmed that relatively high levels of mRNA for GLT-1A are expressed in hepatoma cells compared with negligible levels in rat hepatocytes. To our knowledge, this is the first report of the cloning of a high-affinity glutamate transporter from a transformed cell line and also the first demonstration of functional expression of GLT-1 outside the central nervous system. |
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Authors:
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M Pollard; J McGivan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 484 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2000 Nov |
Date Detail:
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Created Date: 2000-11-17 Completed Date: 2000-12-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 74-6 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, School of Medical Sciences, University Walk, BS8 1TD, Bristol, UK. m.pollard@bristol.ac.uk |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AF297648 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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ATP-Binding Cassette Transporters
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biosynthesis*,
genetics Amino Acid Transport System X-AG Animals Carcinoma, Hepatocellular / metabolism*, pathology Cloning, Molecular DNA, Complementary / analysis Gene Expression Liver Neoplasms / metabolism*, pathology Molecular Sequence Data Rats Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/ATP-Binding Cassette Transporters; 0/Amino Acid Transport System X-AG; 0/DNA, Complementary |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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