Document Detail


A rapidly resorbable hemostatic biomaterial based on dihydroxyacetone.
MedLine Citation:
PMID:  19653301     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have developed a rapid acting, rapidly resorbable, non-toxic, topical hemostatic agent comprised of a PEGylated, polymerized sequence of dihydroxyacetone (MPEG-pDHA) that is highly effective in vivo. Twenty-eight Sprague-Dawley rats underwent left lateral hepatectomy. To the cut edge of the liver, rats received MPEG-pDHA (50 mg), normal saline (0.5 mL), or Instat (50 mg), a commercially available hemostatic compound. Bleeding time and total blood loss were quantified. Coagulation studies and scanning electron microscopy were performed on phlebotomized blood combined with MPEG-pDHA. Rats treated with MPEG-pDHA had significantly decreased bleeding time (97 s) and total blood loss (1.35 g) compared to normal saline (464 s and 3.83 g, p < 0.05 for each), and a significantly shorter bleeding time compared to Instat (165 s, p < 0.05). Histology confirmed that all MPEG-pDHA was metabolized within 3 weeks. The addition of MPEG-pDHA to whole blood did not significantly affect prothrombin time (12.0 s vs. 13.2 s, p = 0.130), partial thromboplastin time (27.0 s vs. 21.8 s, p = 0.118), or thrombin clotting time. MPEG-pDHA is an effective and rapidly resorbable hemostatic agent that may find broad hemostatic application in a wide range of surgical procedures.
Authors:
Peter W Henderson; Daniel J M Kadouch; Sunil P Singh; Peter N Zawaneh; Jennifer Weiser; Sara Yazdi; Andrew Weinstein; Ursula Krotscheck; Bennett Wechsler; David Putnam; Jason A Spector
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  93     ISSN:  1552-4965     ISO Abbreviation:  J Biomed Mater Res A     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-03-25     Completed Date:  2010-06-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  776-82     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Wiley Periodicals, Inc.
Affiliation:
Division of Plastic and Reconstructive Surgery, Laboratory for Bioregenerative Medicine and Surgery, Weill Cornell Medical College, New York, New York, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biocompatible Materials* / chemistry,  metabolism
Blood Coagulation Tests
Dihydroxyacetone* / chemistry,  metabolism
Hemostatics* / chemistry,  metabolism
Hepatectomy
Liver / injuries
Male
Materials Testing
Molecular Structure
Polyethylene Glycols / chemistry,  metabolism
Random Allocation
Rats
Rats, Sprague-Dawley
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Hemostatics; 0/Polyethylene Glycols; 96-26-4/Dihydroxyacetone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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