Document Detail


A rapid technique for producing silver-stained nucleolus organizer regions and trypsin-giemsa bands on human chromosomes.
MedLine Citation:
PMID:  352910     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple and rapid technique is described whereby the nucleolus organizer regions (NORs) of human chromosomes can be differentially stained with silver. This staining is followed by trypsin-Giemsa banding on the same metaphase chromosomes. The metaphases simultaneously exhibit silver-stained NORs and G bands, allowing for the unequivocal identification of all chromosomes and greatly facilitating studies involving the NOR-bearing acrocentrics.
Authors:
W M Howell; D A Black
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Human genetics     Volume:  43     ISSN:  0340-6717     ISO Abbreviation:  Hum. Genet.     Publication Date:  1978 Jul 
Date Detail:
Created Date:  1978-09-30     Completed Date:  1978-09-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7613873     Medline TA:  Hum Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  53-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Azure Stains
Cell Nucleolus*
Chromosomes, Human*
Cytological Techniques
Genes
Genetic Techniques
Humans
Silver*
Chemical
Reg. No./Substance:
0/Azure Stains; 7440-22-4/Silver

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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